Figure 1: Experimental flow of ARE assay systems. (a) Preparation of samples A and B. (b) HepG2-AD13 cell stably transfected with a bioluminescent firefly luciferase gene downstream of ARE-enabled analysis of ARE-mediated gene expression by measuring luminescence. CR: Composite resin, D-MEM: Dulbecco's minimum essential medium. ARE: Antioxidant-responsive element