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Figure 1: Calculations of parameters evaluated by confocal laser scanning microscopy image browser analysis. (a) Gray line near to scale revealed the longest penetration depth of remaining sealer and the percentage of penetrated sealer into the dentinal tubules was calculated as follows: the amount of sealer penetrated in canal perimeter (white line) divided by the total canal perimeter (white lines plus yellow lines) × 100 (%). (b) The percentage of penetration depth of remaining sealer was calculated as follows: the length of the longest penetration depth of the remaining sealer (white line) divided by the full length (green line) from the root canal space to the outer root surface × 100 (%) 
