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  Indian J Med Microbiol
 

Figure 19: Dentin particles packed into bacterial biofilm in an isthmus. A mesial root of a mandibular molar was treated endodontically and clinically, gave a radiographically satisfactory result. The tip of the root was then surgically removed and subjected to transmission electron microscopy. Note the bacterial biofi lm that remained in the isthmus, unaffected by the action of sodium hypochlorite and the dentin particles (arrows) that were pushed into the isthmus by the rotary files (adapted from Nair et al. 2005)[75]

Figure 19: Dentin particles packed into bacterial biofilm in an isthmus. A mesial root of a mandibular molar was treated endodontically and clinically, gave a radiographically satisfactory result. The tip of the root was then surgically removed and subjected to transmission electron microscopy. Note the bacterial biofi lm that remained in the isthmus, unaffected by the action of sodium hypochlorite and the dentin particles (arrows) that were pushed into the isthmus by the rotary files (adapted from Nair <i>et al</i>. 2005)<sup>[75]</sup>