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  Indian J Med Microbiol
 

Figure 2: Detection of Enterococcus faecalis by Polymerase Chain Reaction in the experimentally treated groups and control. P - Positive Marker; N-Negative Marker. (a) Lane 1(G1)-5.25% sodium hypochlorite (NaOCl) +17%Ethylenediaminetetraacetic acid (EDTA) +5.25% NaOCl andDiode Laser. 1-10-negative samples (e-faecalis absent). Lane 2(G2)- 5.25% NaOCl +17% EDTA+5.25% NaOCl. 1-10 positive samples (e-faecalis present). (b) Lane 3(G3)-1.3% NaOCl + MTAD [mixture of doxycycline, citric acid and a detergent (Tween 80)] and Diode Laser. 1-10 negative samples. Lane 4 (G4) -1.3% NaOCl + MTAD. 1-10-positive samples. (c) Lane 5(G5)– Saline and Diode Laser. 1,2,4,6-positive samples. 3, 5, 7, 8, 9, 10-negative samples. (d) Lane 6(G6)-Saline (control)-1-10-positive samples

Figure 2: Detection of <i>Enterococcus faecalis</i> by Polymerase Chain Reaction in the experimentally treated groups and control. P - Positive Marker; N-Negative Marker. (a) Lane 1(G1)-5.25% sodium hypochlorite (NaOCl) +17%Ethylenediaminetetraacetic acid (EDTA) +5.25% NaOCl andDiode Laser. 1-10-negative samples (e-faecalis absent). Lane 2(G2)- 5.25% NaOCl +17% EDTA+5.25% NaOCl. 1-10 positive samples (e-faecalis present). (b) Lane 3(G3)-1.3% NaOCl + MTAD [mixture of doxycycline, citric acid and a detergent (Tween 80)] and Diode Laser. 1-10 negative samples. Lane 4 (G4) -1.3% NaOCl + MTAD. 1-10-positive samples. (c) Lane 5(G5)– Saline and Diode Laser. 1,2,4,6-positive samples. 3, 5, 7, 8, 9, 10-negative samples. (d) Lane 6(G6)-Saline (control)-1-10-positive samples