Journal of Conservative Dentistry

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 23  |  Issue : 3  |  Page : 221--226

Evaluation of the effect of natural versus synthetic matrix metalloproteinase silencers in preservation of dentin collagen and long-term bond strength of total etch adhesive


Megha Nagpal1, Sonali Taneja2, Mohit Kumar2, Chetna Dudeja2 
1 Private Practitioner, New Delhi, India
2 Department of Conservative Dentistry & Endodontics, ITS Dental College and Hospital, Ghaziabad, Uttar Pradesh, India

Correspondence Address:
Dr. Sonali Taneja
Department of Conservative Dentistry and Endodontics, ITS Dental College and Hospital, Muradnagar, Ghaziabad - 201 201, Uttar Pradesh
India

Aim: This study investigated the effect of various synthetic (galardin [Gal] and benzalkonium chloride [BAC]) and natural agents (hesperidin [HES] and epigallocatechin gallate) on the stability of dentin collagen matrix to resist collagenase degradation and improve long-term microtensile bond strength. Materials and Methods: Ten sound-impacted third molars were collected and manual removal of pulp, periodontal ligament, cementum, and enamel was done. Remaining dentin fragments were pulverized under liquid nitrogen to obtain dentin powder. 2 mg aliquot of dentin powder was allocated to each of the test solutions and subjected to hydroxyproline assay. Another 60 sound human third molars were collected and occlusal enamel was ground flat to reach dentinoenamel junction. Class I cavities were prepared in dentin, followed by etching using 37% phosphoric acid for 15 s. Samples were then subjected to surface treatment with different agents for 60 s, followed by application of Optibond S and restoration with P 60 composite resin. Samples of all groups except control were subject to thermocycling. Samples were sectioned to 1 mm thick slabs which were subject to universal testing machine to determine ultimate tensile strength. One-way analysis of variance and Bonferroni post hoc test with a significance level of P < 0.05 were used to analyze data. Results: HES resulted in maximum resistance to collagen degradation, followed by epigallocatechin gallate (EGCG), Gal, and BAC with a significant difference among the groups. Samples of Gal group showed the highest microtensile bond strength values, followed by HES, EGCG, BAC with a significant difference between the groups except HES and EGCG where the difference was nonsignificant. Conclusion: The use of matrix metalloproteinase silencers could improve the mechanical properties of collagen and resist enzymatic degradation, leading to an improved long-term intimate restoration.


How to cite this article:
Nagpal M, Taneja S, Kumar M, Dudeja C. Evaluation of the effect of natural versus synthetic matrix metalloproteinase silencers in preservation of dentin collagen and long-term bond strength of total etch adhesive.J Conserv Dent 2020;23:221-226


How to cite this URL:
Nagpal M, Taneja S, Kumar M, Dudeja C. Evaluation of the effect of natural versus synthetic matrix metalloproteinase silencers in preservation of dentin collagen and long-term bond strength of total etch adhesive. J Conserv Dent [serial online] 2020 [cited 2021 Jan 20 ];23:221-226
Available from: https://www.jcd.org.in/article.asp?issn=0972-0707;year=2020;volume=23;issue=3;spage=221;epage=226;aulast=Nagpal;type=0