Journal of Conservative Dentistry

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 22  |  Issue : 6  |  Page : 538--543

Chemical constituent, minimal inhibitory concentration, and antimicrobial efficiency of essential oil from oreganum vulgare against Enterococcus faecalis: An in vitro study


Krishnamachari Janani, P Ajitha, Raghu Sandhya, Kavalipurapu Venkata Teja 
 Department of Conservative Dentistry and Endodontics, Saveetha Institute of Medical and Technical Sciences, Saveetha Dental College, Chennai, Tamil Nadu, India

Correspondence Address:
Prof. P Ajitha
Department of Conservative Dentistry and Endodontics, Saveetha Institute of Medical and Technical Sciences, Saveetha Dental College, 162, Poonamallee High Road, Chennai - 600 077, Tamil Nadu
India

Aim: The purpose of this study was to determine the chemical composition of oregano essential oil, minimal inhibitory concentration (MIC) and to assess its antimicrobial efficiency against Enterococcus faecalis. Material and Methods: Gas Chromatography and Mass Spectrometry (GC-MS) was used to determine the chemical composition of essential oil from oreganum vulgare. Broth dilution and agar diffusion method was used to evaluate the MIC. For Broth dilution, 100 μL of different concentration of oil (6.25, 12.5, 25.0, 50.0, and 100 μg/ml) was tested. Agar diffusion method was utilized to evaluate the antimicrobial efficiency of different concentration of oil (25.0, 50.0, and 100 μg/mL) against E. faecalis. Results: GC-MS analysis revealed that oregano essential oil contained carvacrol (41.2%), γ-terpinene (12.68%), p-cymene (9.47%), α-terpinene (1.19%) as the major compounds and β–caryophyllene (0.83%), β-linalool (0.67%), β–bisabolene (0.601%), α-pinene (0.6%), β-pinene (0.5%), terpinen-4-ol (0.41%), borneol (0.4%), 3-thujene (0.4%), spathulenol (0.4%), myristicin (0.25%), and apiol (0.14%). The results of the present study reported Oregano essential oil possess antimicrobial activity against E. faecalis. The MIC was 25 μg/ml and the minimum bacterial concentration (MBC) was 50 μg/ml. Conclusion: Oregano essential oil was reported to be an effective antimicrobial agent against E. faecalis. The MIC was found to be 25 μg/ml and the MBC was found to be 50 μg/ml.


How to cite this article:
Janani K, Ajitha P, Sandhya R, Teja KV. Chemical constituent, minimal inhibitory concentration, and antimicrobial efficiency of essential oil from oreganum vulgare against Enterococcus faecalis: An in vitro study.J Conserv Dent 2019;22:538-543


How to cite this URL:
Janani K, Ajitha P, Sandhya R, Teja KV. Chemical constituent, minimal inhibitory concentration, and antimicrobial efficiency of essential oil from oreganum vulgare against Enterococcus faecalis: An in vitro study. J Conserv Dent [serial online] 2019 [cited 2020 Oct 31 ];22:538-543
Available from: https://www.jcd.org.in/article.asp?issn=0972-0707;year=2019;volume=22;issue=6;spage=538;epage=543;aulast=Janani;type=0