Year : 2018 | Volume
: 21 | Issue : 1 | Page : 42--46
Antibacterial activity of dental restorative material: An in vitro study
Nishmitha N Hegde, Shruthi H Attavar, Mithra N Hegde, G Priya
Department of Conservative Dentistry and Endodontics, A. B. Shetty Memorial Institute of Dental Sciences, Nitte University, Deralakatte, Mangaluru, Karnataka, India
Aim: The study evaluated the antibacterial effects of dental restorations such as nano-hybrid composite (Empress), a glass ionomer cement (GIC Gold label Type 9) and silver amalgam against Streptococcus mutans.
Materials and Methods: A modified bacterial suspension within the material assay was used to study the antibacterial effects. A volume of 20 μl of bacterial suspensions were placed in a narrow conical cavity within the materials. They were incubated for 0, 3, 6, 12, 24, and 48 h at 37°C. After the incubation period, the number of viable cells in the suspension was evaluated. In liquid culture assay, growth inhibition was measured at A600 nm up to 6 h in bacterial suspensions treated with the eluates of the restorative materials.
Statistical Analysis: Kruskal–Wallis test and Mann–Whitney's test were performed to determine the significant differences between the control and restorative materials for given incubation periods at 5% level of significance (P < 0.05)
Results and Conclusion: After 6 h of incubation, all restorative materials showed an inhibitory effect when compared to the controls. Silver amalgam showed the highest inhibition, followed by GIC Type 9 and composite. Silver amalgam showed marked inhibition after 2 h in comparison to the other material groups. These techniques employed to study the antibacterial effects showed that the silver amalgam had the pronounced inhibitory effect followed by GIC and composite. Further research on these aspects is necessary to determine whether the material can prevent secondary caries formation.
Dr. Mithra N Hegde
Vice Dean and Head of the Department of Conservative Dentistry and Endodontics, A. B. Shetty Memorial Institute of Dental Sciences, Nitte (Deemed to be University), Mangaluru- 575 018, Karnataka
|How to cite this article:|
Hegde NN, Attavar SH, Hegde MN, Priya G. Antibacterial activity of dental restorative material: An in vitro study.J Conserv Dent 2018;21:42-46
|How to cite this URL:|
Hegde NN, Attavar SH, Hegde MN, Priya G. Antibacterial activity of dental restorative material: An in vitro study. J Conserv Dent [serial online] 2018 [cited 2022 Jan 20 ];21:42-46
Available from: https://www.jcd.org.in/article.asp?issn=0972-0707;year=2018;volume=21;issue=1;spage=42;epage=46;aulast=Hegde;type=0