Journal of Conservative Dentistry

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 18  |  Issue : 2  |  Page : 159--162

Evaluation of minimal inhibitory concentration of two new materials using tube dilution method: An in vitro study


Geeta S Hiremath1, Raghavendra D Kulkarni2, Balaram D Naik1 
1 Department of Conservative Dentistry and Endodontics, Sri Dharmasthala Manjunatheshwara College of Medical Sciences and Hospital, Dharwad, Karnataka, India
2 Department of Microbiology, Sri Dharmasthala Manjunatheshwara College of Medical Sciences and Hospital, Dharwad, Karnataka, India

Correspondence Address:
Geeta S Hiremath
Departments of Conservative Dentistry and Endodontics, SDM College of Dental Sciences and Hospital, Dharwad - 580 001, Karnataka
India

Aim: The aim of the study is to evaluate and compare the antimicrobial efficacy of two new materials MTA Plus and Biodentine with ProRoot MTA using tube dilution method. Materials and Methods: The materials used were ProRoot MTA (Dentsply), MTA Plus (compounded by Prevest Denpro, Jammu, India for Avalon Biomed Inc, USA) and a calcium silicate based material Biodentine (Septodont, Saint-Maur-des-Fosses, France). Doubling dilutions of the material were prepared in Sabouraud«SQ»s dextrose broth (SDB) and Brain Heart Infusion (BHI) broth for Candida albicans and Enterococcus faecalis, respectively. The minimal concentration at which inhibition of microorganism occurred was measured and noted as minimal inhibitory concentration (MIC) of the material. Results: There was no statistically significant difference between the materials against C. albicans. Biodentine was statistically significant than MTA Plus against E. faecalis (P-value-0.022). ProRoot MTA was statistically significant at different time intervals against E. faecalis (P-value-0.001). Conclusion: ProRoot MTA and Biodentine proved to have antimicrobial property. MTA Plusproved as a good antifungal agent.


How to cite this article:
Hiremath GS, Kulkarni RD, Naik BD. Evaluation of minimal inhibitory concentration of two new materials using tube dilution method: An in vitro study .J Conserv Dent 2015;18:159-162


How to cite this URL:
Hiremath GS, Kulkarni RD, Naik BD. Evaluation of minimal inhibitory concentration of two new materials using tube dilution method: An in vitro study . J Conserv Dent [serial online] 2015 [cited 2021 Feb 25 ];18:159-162
Available from: https://www.jcd.org.in/article.asp?issn=0972-0707;year=2015;volume=18;issue=2;spage=159;epage=162;aulast=Hiremath;type=0