Journal of Conservative Dentistry
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ORIGINAL ARTICLE
Year : 2020  |  Volume : 23  |  Issue : 5  |  Page : 479-483

Evaluation of cell viability of human dental pulp stem cells in two dimensional and three dimensional fibrin glue scaffold


1 Department of Conservative Dentistry and Endodontics, Government Dental College, Ahmedabad, Gujarat, India
2 Department of Biotechnology, Institute of Science, Nirma University, Ahmedabad, Gujarat, India

Correspondence Address:
Dr. Needa A Ansari
B-18, Rehnuma Society, Near F. D. School, Sarkhej Road, Ahmedabad, Gujarat
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/JCD.JCD_439_20

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Context: Regenerative endodontics uses the concept of tissue engineering to restore the diseased immature tooth, allowing for continued development of the root to a healthy state. For regenerative endodontics, the use of human dental pulp stem cells (HDPSCs) with appropriate scaffolds and growth factors is imperative. Aims: The aim of the study was to evaluate the human dental pulp cell viability in two-dimensional (2D) and 3D fibrin glue scaffold to be used in regenerative endodontics. Subjects and Methods: Regenerative potential of HDPSCs was comparatively assessed usings 2D and 3D fibrin glue scaffold. 3D scaffold was made with different concentrations of fibrinogen. Cell morphology was studied under inverted phase-contrast microscopy, and cell proliferation was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay at various time intervals. Statistical Analysis Used: Collected data underwent a two-way ANOVA test. The P value of the study was kept 0.05 according to the sample size. Results: Study revealed a significant increase in the proliferation of HDPSCs in fibrin glue precoated wells of 2D fibrin glue compared to preseeded cells on day 1 and day 3. The concentration of fibrinogen has a major role in cell viability in 3D fibrin glue scaffold. Homing of HDPSCs in the 3D scaffold improves with time. Conclusions: This study concludes that the concentration of fibrin glue has a significant role in HDPSC Viability in 3D scaffold.


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