Journal of Conservative Dentistry
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Year : 2020  |  Volume : 23  |  Issue : 3  |  Page : 314-318

Comparative evaluation of apical extrusion of intracanal bacteria using ProTaper Next, Mtwo, and ProTaper rotary systems: An in vitro study

1 Department of Dentistry, All India Institute of Medical Sciences, Mangalagiri, Andhra Pradesh, India
2 Department of Conservative Dentistry and Endodontics, Anil Neerukonda Institute of Dental Sciences, Vishakhapatnam, Andhra Pradesh, India
3 Department of Conservative Dentistry and Endodontics, Sibar Institute of Dental Sciences, Guntur, Andhra Pradesh, India
4 Department of Conservative Dentistry and Endodontics, CKS Theja Institute of Dental Sciences, Tirupati, Andhra Pradesh, India
5 Department of Conservative Dentistry and Endodontics, Konaseema Institute of Dental Sciences, Amalapuram, Andhra Pradesh, India

Correspondence Address:
Dr. Swapnika Polineni
Door No: 6-4-4, 4/5 Arundel Pet, Opposite Help Hospital, Guntur - 522 002, Andhra Pradesh
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/JCD.JCD_283_19

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Aim: The aim of this study was to evaluate the number of intracanal bacteria extruded apically after instrumentation with three different nickel–titanium rotary instruments. Materials and Methodology: Forty freshly extracted mandibular premolars were selected, access cavities were prepared, and the teeth were mounted in the bacterial collection apparatus. Root canals were contaminated with a suspension of Enterococcus faecalis and incubated for 24 h at 37°C. The contaminated teeth were divided into four groups of 10 teeth each according to the rotary system used for instrumentation: Group 1: ProTaper universal files, Group 2: MTwo files, Group 3: ProTaper Next files, and Group 4: Control group (no instrumentation). Bacteria extruded after preparations were collected into vials. The number of colony-forming units (CFUs) was determined for each sample. Statistical Analysis: The data obtained were analyzed using the one-way analysis of variance followed by post hoc Tukey’s test with a P = 0.05 as the level for statistical significance. Results: The results suggested a statistically significant difference in the number of CFUs between four experimental groups (P < 0.001). Conclusion: Least amount of bacterial extrusion was seen in ProTaper Next Group while more bacterial extrusion was seen in MTwo Group.

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