Development of a dual-cure mineral trioxide aggregate-based cement: Biological, physical, and mechanical properties
Laura Siqueira Pintado1, Eliana do Nascimento Torre1, Maicon dos Santos Selayaran1, Rodrigo Varella de Carvalho2, Cesar Henrique Zanchi3, Fábio Renato Manzolli Leite4, Adriana Etges1
1 Department of Semiology and Clinics, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil
2 Department of Restorative Dentistry, School of Dentistry, IMED Faculdade Meridional, Passo Fundo, RS, Brazil
3 Biomaterials Development and Control Center, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil
4 Department of Semiology and Clinics, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil; Department of Dentistry and Oral Health, Section of Periodontology, Aarhus University, Aarhus, Denmark
Dr. Fábio Renato Manzolli Leite
Department of Dentistry and Oral Health, Section of Periodontology Aarhus University, Vennelyst Boulevard 9, Aarhus, 8000
Source of Support: None, Conflict of Interest: None
Purpose: The purpose of this study is to compare the physical, mechanical, and biocompatibility properties of a new dual-cure white mineral trioxide aggregate (D-W-MTA) and a commercial W-MTA.
Materials and Methods: Diametral tensile strength (DTS), water sorption (WSp), and water solubility (WSl) tests were performed. Cytotoxicity was observed in primary culture of human pulp fibroblasts (HPFs) and mouse 3T3/NIH fibroblast lineage. Specimens of both materials were embedded in 1 mL of Dulbecco's modified essential medium for 24 h. Cells were incubated for 24 h with the eluates. Cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and genotoxicity by micronucleus (MN) formation. Data were analyzed by ANOVA and Kruskal–Wallis tests considering P < 0.05.
Results: D-MTA and W-MTA not showed cytotoxic effect on the two cell lines. However, D-MTA stimulated HPF growth. The MN count was similar to that of the control group for D-MTA and W-MTA. D-MTA presented lower DTS and WSl. Nevertheless, WSp was similar in the two groups.
Conclusion: The results suggest that D-MTA is a promising material for pulp capping. Thus, in vivo tests should be performed to evaluate the performance of this material.