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Development of a dual-cure mineral trioxide aggregate-based cement: Biological, physical, and mechanical properties
Laura Siqueira Pintado1, Eliana do Nascimento Torre1, Maicon dos Santos Selayaran1, Rodrigo Varella de Carvalho2, Cesar Henrique Zanchi3, Fábio Renato Manzolli Leite4, Adriana Etges1
1 Department of Semiology and Clinics, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil
2 Department of Restorative Dentistry, School of Dentistry, IMED Faculdade Meridional, Passo Fundo, RS, Brazil
3 Biomaterials Development and Control Center, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil
4 Department of Semiology and Clinics, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil; Department of Dentistry and Oral Health, Section of Periodontology, Aarhus University, Aarhus, Denmark
Correspondence Address:
Dr. Fábio Renato Manzolli Leite
Department of Dentistry and Oral Health, Section of Periodontology Aarhus University, Vennelyst Boulevard 9, Aarhus, 8000
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/JCD.JCD_393_16
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Purpose: The purpose of this study is to compare the physical, mechanical, and biocompatibility properties of a new dual-cure white mineral trioxide aggregate (D-W-MTA) and a commercial W-MTA.
Materials and Methods: Diametral tensile strength (DTS), water sorption (WSp), and water solubility (WSl) tests were performed. Cytotoxicity was observed in primary culture of human pulp fibroblasts (HPFs) and mouse 3T3/NIH fibroblast lineage. Specimens of both materials were embedded in 1 mL of Dulbecco's modified essential medium for 24 h. Cells were incubated for 24 h with the eluates. Cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and genotoxicity by micronucleus (MN) formation. Data were analyzed by ANOVA and Kruskal–Wallis tests considering P < 0.05.
Results: D-MTA and W-MTA not showed cytotoxic effect on the two cell lines. However, D-MTA stimulated HPF growth. The MN count was similar to that of the control group for D-MTA and W-MTA. D-MTA presented lower DTS and WSl. Nevertheless, WSp was similar in the two groups.
Conclusion: The results suggest that D-MTA is a promising material for pulp capping. Thus, in vivo tests should be performed to evaluate the performance of this material. |
