| ORIGINAL ARTICLE |
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| Year : 2018 | Volume
: 21
| Issue : 1 | Page : 21-25 |
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Cytotoxicity of gypsum-based biomaterial for direct pulp capping using stem cells from human exfoliated deciduous teeth
Hasan Subhi1, Fazal Reza2, Adam Husein1, Asma Abdullah Nurul3
1 Conservative Dentistry Unit, School of Dental Sciences, Universiti Sains , 16150 Kubang Kerian, Kelantan, Malaysia
2 Biomaterials and Prosthodontics Unit, School of Dental Sciences, Universiti Sains , 16150 Kubang Kerian, Kelantan, Malaysia
3 School of Health Sciences, Universiti Sains , 16150 Kubang Kerian, Kelantan, Malaysia
Correspondence Address:
Dr. Asma Abdullah Nurul
School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan
Malaysia
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/JCD.JCD_86_17
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Aim: The aim of this study was to evaluate the cytotoxicity effects of experimental gypsum-based biomaterial prepared with various concentrations of chitosan (Gyp-CHT).
Materials and Methods: The study was performed using cell viability assay for mitochondrial dehydrogenase activity in stem cells from human exfoliated deciduous teeth (SHED), after 1, 2, and 3 days of exposure to the biomaterial extracts of varying concentrations. Differences in mean cell viability values were assessed by one-way analysis of variance, followed by Dunnett T3 post hoc test for multiple comparisons (P < 0.05).
Results: The cell viability to Gyp-CHT in low extract concentrations was statistically similar to that of the control and different from that of high extract concentrations. Gyp-5% CHT showed the highest percentage of cell viability with 110.92%, 108.56%, and 109.11%. The cell viability showed a tendency toward increment with low extract concentration and no constant effect of CHT on cell viability toward higher or lower.
Conclusions: Gyp-CHT biomaterial has no cytotoxic effects on the cultured SHED. |
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