Journal of Conservative Dentistry
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Year : 2017  |  Volume : 20  |  Issue : 5  |  Page : 341-345

Effect of human, dentin, albumin and lipopolysaccharide on the antibacteerial activity of endodontic activity of endodontic irrigants

1 Graduate program in dentistry, Faculty of Dentistry, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil
2 Department of Social and Preventive Dentistry, Faculty of Dentstry, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil
3 Department of Stomatology, Federal University of Santa Maria, Santa Maria, RS, Brazil

Correspondence Address:
Dr. Patrícia Maria Poli Kopper
Department of Conservative Dentistry, Faculty of Dentistry, Federal University of Rio Grande Do Sul, Ramiro Barcelos St. 2492. Porto Alegre, RS
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/JCD.JCD_129_16

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Context: Human dentin powder (HD), bovine serum albumin (BSA) and endotoxin (LPS) may affect the antimicrobial activity of irrigating solutions. Aim: To evaluate the inhibitory effect of HD powder, BSA, and LPS on the antibacterial activity of 0.5%, 1%, 2.5%, and 5% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) gel, BioPure mixture of tetracycline, citric acid, and detergent (MTAD), and QMix. Methods: The direct contact test against Enterococcus faecalis (ATCC 29212) for 2-min, 30-min and 6-h was used. Sterile pyrogen-free water was the negative control. After experimental periods, a neutralizing agent was used. Colony-forming units were determined by 10-fold serial dilutions and culture on agar plates. Data were analyzed by Kruskal–Wallis and Dunn's test (α = 5%). Results: In the absence of inhibitors, all irrigants eliminated E. faecalis. In contact with HD, all solutions eliminated E. faecalis within 2-min, with the exception of MTAD. In the presence of BSA, only 5% NaOCl killed E. faecalis within 2-min. LPS did not affect the antibacterial effect of any irrigant. At 30-min and at 6-h, all substances eliminated E. faecalis. Conclusions: In the presence of albumin, irrigants needed >2-min to eliminate E. faecalis, except for 5% NaOCl. The same was observed in the presence of dentin when E. faecalis was exposed to MTAD.

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