| ORIGINAL RESEARCH ARTICLE |
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| Year : 2017 | Volume
: 20
| Issue : 5 | Page : 341-345 |
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Effect of human, dentin, albumin and lipopolysaccharide on the antibacteerial activity of endodontic activity of endodontic irrigants
Ramiro Martins Quintana1, Alexander Pompermayer Jardine1, Francisco Montagner1, Clarissa Cavalcanti Fatturi Parolo2, Renata Dornelles Morgental3, Patrícia Maria Poli Kopper1
1 Graduate program in dentistry, Faculty of Dentistry, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil
2 Department of Social and Preventive Dentistry, Faculty of Dentstry, Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil
3 Department of Stomatology, Federal University of Santa Maria, Santa Maria, RS, Brazil
Correspondence Address:
Dr. Patrícia Maria Poli Kopper
Department of Conservative Dentistry, Faculty of Dentistry, Federal University of Rio Grande Do Sul, Ramiro Barcelos St. 2492. Porto Alegre, RS
Brazil
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/JCD.JCD_129_16
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Context: Human dentin powder (HD), bovine serum albumin (BSA) and endotoxin (LPS) may affect the antimicrobial activity of irrigating solutions.
Aim: To evaluate the inhibitory effect of HD powder, BSA, and LPS on the antibacterial activity of 0.5%, 1%, 2.5%, and 5% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) gel, BioPure mixture of tetracycline, citric acid, and detergent (MTAD), and QMix.
Methods: The direct contact test against Enterococcus faecalis (ATCC 29212) for 2-min, 30-min and 6-h was used. Sterile pyrogen-free water was the negative control. After experimental periods, a neutralizing agent was used. Colony-forming units were determined by 10-fold serial dilutions and culture on agar plates. Data were analyzed by Kruskal–Wallis and Dunn's test (α = 5%).
Results: In the absence of inhibitors, all irrigants eliminated E. faecalis. In contact with HD, all solutions eliminated E. faecalis within 2-min, with the exception of MTAD. In the presence of BSA, only 5% NaOCl killed E. faecalis within 2-min. LPS did not affect the antibacterial effect of any irrigant. At 30-min and at 6-h, all substances eliminated E. faecalis.
Conclusions: In the presence of albumin, irrigants needed >2-min to eliminate E. faecalis, except for 5% NaOCl. The same was observed in the presence of dentin when E. faecalis was exposed to MTAD. |
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