| ORIGINAL ARTICLE |
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| Year : 2016 | Volume
: 19
| Issue : 2 | Page : 134-137 |
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Antimicrobial effect of three disinfecting agents on Resilon cones and their effect on surface topography: An in vitro study
Mahesh Martur Chandrappa, Prasanna Mahadevasa Meharwade, Raghu Srinivasan, Shreetha Bhandary, Farhat Nasreen
Department of Conservative Dentistry and Endodontics, AECS Maaruti College of Dental Sciences and Research Centre, Bangalore, Karnataka, India
Correspondence Address:
Prasanna Mahadevasa Meharwade
House No. 21/4760, Sirur Park, 1st Stage, 4th Main Vidyanagar, Hubli - 580 021, Karnataka
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0972-0707.178689
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Aim: The objective of this study was to evaluate the effectiveness of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and 2% peracetic acid (PAA) in disinfecting Resilon cones and to evaluate topographical changes microscopically under scanning electron microscope (SEM) after rapid chemical disinfection.
Materials and Methods: Resilon cones were disinfected in an ultraviolet (UV) light chamber for 20 min and contaminated by immersing in a microbial suspension of Enterococcus faecalis for 30 min. The contaminated cones were then immersed in the 5.25% NaOCl, 2% CHX, and 2% PAA for 1 min, 5 min, and 10 min, separately. The cones were then incubated at 37C in thioglycollate broth for 7 days and examined for turbidity. The samples showing turbidity were subcultured on blood agar and incubated at 37C for 48 h. Gram staining was done to confirm that the cultured bacteria were E. faecalis. Surface changes of disinfected Resilon cones were evaluated under SEM.
Statistical Analysis Used: The data were analyzed statistically using Kruskal-Wallis, analysis of variance (ANOVA), and Mann-Whitney U-test.
Result: In eliminating E. faecalis, 5.25% NaOCl was most effective followed by 2% PAA and 2% CHX. Topographic examination of tested Resilon cones revealed some surface deposits after disinfection with 5.25% NaOCl and 2% CHX, whereas 2% PAA caused surface erosion.
Conclusion: In disinfecting Resilon cones, 5.25% NaOCl is most effective followed by 2% PAA and 2% CHX. |
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