Journal of Conservative Dentistry
Home About us Editorial Board Instructions Submission Subscribe Advertise Contact e-Alerts Login 
Users Online: 103
Print this page  Email this page Bookmark this page Small font sizeDefault font sizeIncrease font size
 

 
Table of Contents   
ORIGINAL ARTICLE  
Year : 2015  |  Volume : 18  |  Issue : 5  |  Page : 360-363
Effect of bromelain enzyme for dentin deproteinization on bond strength of adhesive system


Department of Conservative Dentistry and Endodontics, College of Dental Sciences, Davangere, Karnataka, India

Click here for correspondence address and email

Date of Submission27-Apr-2015
Date of Decision22-Jun-2015
Date of Acceptance02-Jul-2015
Date of Web Publication1-Sep-2015
 

   Abstract 

Aims: To assess the deproteinizing effect of bromelain enzyme and compare it with 5% sodium hypochlorite (NaOCl) on shear bond strength before application of the adhesive system.
Materials and Methods: A total of 30 extracted human premolars were divided into three groups, each one consisted of 10 teeth. The occlusal surface was wet ground to expose superficial dentin. In Group 1, teeth were etched; in Group 2, teeth were etched and deproteinized with bromelain enzyme; in Group 3, teeth were etched and deproteinized with 5% NaOCl. Upon completion of the adhesive procedures, resin composite was inserted into the plastic tube and light-polymerized. All specimens were stored at 37°C in water for 24 h, and the specimens were transferred to the universal testing machine, and then subjected to shear bond strength analysis at a crosshead speed of 1.0 mm/min.
Statistical Analysis Used: Data were statistically analyzed using one-way analysis of variance and unpaired t-test at a significance level of 0.05. The statistical analysis was performed using SPSS version 12.0.1 for Windows (SPSS Inc., Chicago, IL, USA).
Results: The bond strength results were significantly influenced by the application of bromelain enzyme. Statistically significant differences were not demonstrated in control group and NaOCl-treated group. The highest bond strength was seen in bromelain enzyme-treated group.
Conclusions: Within the limitations of the present study, it was concluded that removal of unsupported collagen fiber with bromelain enzyme after acid etching results in improved bond strength.

Keywords: Bromelain enzyme; shear bond strength; sodium hypochlorite

How to cite this article:
Chauhan K, Basavanna RS, Shivanna V. Effect of bromelain enzyme for dentin deproteinization on bond strength of adhesive system. J Conserv Dent 2015;18:360-3

How to cite this URL:
Chauhan K, Basavanna RS, Shivanna V. Effect of bromelain enzyme for dentin deproteinization on bond strength of adhesive system. J Conserv Dent [serial online] 2015 [cited 2023 Oct 1];18:360-3. Available from: https://www.jcd.org.in/text.asp?2015/18/5/360/164029

   Introduction Top


The creation of a hybrid layer or resin-infiltrated dentin-layers (RIDLs) is considered as the most efficient mechanism of adhesion in recent dentin bonding agents (DBAs). [1],[2]

However, controversy exists regarding the role of collagen fibrils on resin adhesion and sealing efficiency of DBAs. Many restorations bonded with different DBAs have shown microleakage. [3] Nanoleakage, a microscopic leakage inside the thickness of RIDL, was first described by Sano et al., collagen matrix degradation occurs either by the breakdown of the polymer phase or collagen fibrils in the hybrid layer. Also, exposure of collagen matrix of dentin by acid etching may activate matrix metalloproteinase (MMP). [4] In order to prevent this biodegradation, various techniques have been employed such as the demineralized collagen removal [5],[6] and the use of MMPs inhibitors. [7]

The use of sodium hypochlorite (NaOCl) to deproteinize acid-etched dentin has several disadvantages. It forms a fragility zone and is cytotoxic with a bad taste and odor. As the dentinal depth increases, the negative impact of NaOCl increases. These disadvantages have led to the search for better means to deproteinized dentin. [8]

Newer techniques for removing collagen network include deproteinizing enzymes such as collagenase or bromelain enzyme. [8]

Bromelain is a proteolytic enzyme (proteases) which belongs to a group of protein-digesting enzymes obtained commercially from the fruit or stem of pineapple. The function of proteases is to catalyze the hydrolysis of proteins to give amino acids. [9]

Bromelain enzyme can reduce nanoleakage after collagen removal as compared to NaOCl [8] but there has not been a study to see its effectiveness in improving the bond strength.

Therefore, the aim of this study was to assess the deproteinizing effect of bromelain enzyme and compare it with 5% NaOCl on shear bond strength before application of the adhesive system.


   Materials and Methods Top


Thirty extracted human premolar teeth were taken and stored in 0.1% thymol solution until they were subjected to use. Roots were embedded in self-cure acrylic resin. Samples were wet ground on the occlusal surface using a series of silicon carbide discs to prepare flat superficial dentin. Teeth were divided into three groups based on the method of dentin deproteinization.

Group 1: Teeth were etched with 37% phosphoric acid (Scotchbond Multi-purpose Etchant, 3M) for 15 s and then rinsed with water and blot dried.

Group 2: Teeth were etched with 37% phosphoric acid for 15 s, rinsed with water, blot dried and deproteinized with bromelain enzyme (Bangalore Sales Corporation) for 1 min. The bromelain enzyme was washed off with distilled water.

Group 3: Teeth were etched with 37% phosphoric acid for 15 s, rinsed with water, blot dried and deproteinized with 5% NaOCl (Nice Chemicals Pvt. Ltd.) for 1 min. NaOCl was washed off with distilled water.

Fifth generation DBA, Adper single bond 2 (3M ESPE), was applied according to manufacturer's instructions. Upon completion of the adhesive procedures, standardized plastic tubes were placed onto the dentin surface. The resin composite Filtek Z-250 (3M ESPE) was inserted into the plastic tube and light-polymerized. The plastic tube was removed to expose the resin cylinder. All specimens were stored at 37°C in water for 24 h before testing, to simulate the oral environment. After storage, the specimens were transferred to the universal testing machine individually and then subjected to shear bond strength analysis at a crosshead speed of 1.0 mm/min.

Statistical analysis

Data were statistically analyzed using one-way analysis of variance for mean comparison among groups and unpaired t-test for mean comparison of shear bond strength between groups at a significance level of 0.05. The statistical analysis was performed using SPSS version 12.0.1 for Windows (SPSS Inc., Chicago, IL, USA).


   Results Top


The bond strength results were significantly influenced by the application of bromelain enzyme (P < 0.05) as shown in [Table 1]. Statistically significant differences were not demonstrated in control group and NaOCl treated group as shown in [Table 2]. The highest bond strength was seen in bromelain enzyme-treated group.
Table 1: Mean value of shear bond strength in MPa of all three tested groups (n = 30)


Click here to view
Table 2: Mean comparison of shear bond strength (MPa) between groups


Click here to view



   Discussion Top


Adhesion to tooth structure depends on various factors including the type of the adhesive system, tooth structure, load cycling, and surface contaminations. [10] Inadequate adhesion of composite resin restorations to dentin results in reduced retention, microleakage, and finally recurrent caries. [11] Progressive loss of bond strength of etch-and-rinse adhesives has been demonstrated in some studies. [12],[13] One of the factors that is responsible for this degradation is incomplete infiltration of resin monomers into unsupported collagen networks after acid etching with strong acids which produces a zone of collagen without any support of either minerals or resins in the base of the hybrid layer. [12]

Questions have been raised whether bond strength tests can predict the clinical behavior of adhesively bonded composite resin restorations since they do not represent the complex clinical failure mechanism. In micro tensile tests, the fracture starts at the weakest part of the bond. A disadvantage of these tests is the high technique sensitivity. [14] In shear test, the fracture does not start at the weakest part of the bond, but always at the insertion point of the load. [15] The preference for the conventional shear test instead of micro shear test is justified because they are easy to perform, requiring minimal equipment, and specimen preparation. [14] Also, a comparative bond strength study involving three adhesive systems has shown similarities between "macro" and "micro" counterparts regarding material ranking. [14]

Numerous studies have evaluated the effects of NaOCl on adhesion process, and different results have been achieved. [16],[17],[18] Some studies, however, have shown lower bond strength using NaOCl. [17] This decrease in bond strength can be attributed to the generation of oxygen after the disintegration of NaOCl into NaCl and O 2 . The released oxygen in this chemical reaction prevents the polymerization of adhesive agents. These reactive residual free radicals in NaOCl-treated dentin compete with the propagation of vinyl-free radicals generated during light activation of the adhesive system, resulting in premature chain termination and incomplete polymerization. [19]

However, NaOCl may exert different effects on bond strength depending on the chemical structure of the adhesive system and the type of the initiator in the adhesive system used. [10]

In this present study, bromelain enzyme performed better which could be because of reduced nanoleakage as shown by the previous study. It has better effectiveness in removing unsupported collagen matrix as compared to NaOCl, and lower nanoleakage is seen. [8]

This could be because of the depletion of collagen from the surface of acid-etched dentin resulting in increased permeability of dentin substrate due to the enlargement of dentinal tubules near the outer dentin surface. This enhances the spreading and diffusing of adhesive monomers through dentin. [20],[21] The surface energy of the dentin is improved, because the hydroxyapatite has a high surface energy substrate while collagen has a low energy surface, and this leads to enhanced diffusion of adhesive monomers through dentin. [22],[23] Also, the dentin is very porous and rough with many lateral branches of tubules which are detectable in main tubules, which may contribute to the increase in the spreading of adhesive monomers through dentin. [24],[25]


   Conclusion Top


Within the limitations of the present study, it was concluded that removal of unsupported collagen fibers with bromelain enzyme after acid etching results in improved bond strength.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

 
   References Top

1.
Nakabayashi N, Kojima K, Masuhara E. The promotion of adhesion by the infiltration of monomers into tooth substrates. J Biomed Mater Res 1982;16:265-73.  Back to cited text no. 1
[PUBMED]    
2.
Barkmeier WW, Los SA, Triolo PT Jr. Bond strengths and SEM evaluation of Clearfil Liner Bond 2. Am J Dent 1995;8:289-93.  Back to cited text no. 2
    
3.
Tay FR, Gwinnett AJ, Pang KM, Wei SH. Variability in microleakage observed in a total-etch wet-bonding technique under different handling conditions. J Dent Res 1995;74:1168-78.  Back to cited text no. 3
    
4.
Sano H, Takatsu T, Ciucchi B, Horner JA, Matthews WG, Pashley DH. Nanoleakage: Leakage within the hybrid layer. Oper Dent 1995;20:18-25.  Back to cited text no. 4
    
5.
Perdigão J, Thompson JY, Toledano M, Osorio R. An ultra-morphological characterization of collagen-depleted etched dentin. Am J Dent 1999;12:250-5. Erratum in: Am J Dent 1999;12:308.  Back to cited text no. 5
    
6.
Uceda-Gómez N, Loguercio AD, Moura SK, Grande RH, Oda M, Reis A. Long-term bond strength of adhesive systems applied to etched and deproteinized dentin. J Appl Oral Sci 2007;15:475-9.  Back to cited text no. 6
    
7.
Osorio R, Yamauti M, Osorio E, Ruiz-Requena ME, Pashley D, Tay F, et al. Effect of dentin etching and chlorhexidine application on metalloproteinase-mediated collagen degradation. Eur J Oral Sci 2011;119:79-85.  Back to cited text no. 7
    
8.
Dayem RN, Tameesh MA. A new concept in hybridization: Bromelain enzyme for deproteinizing dentin before application of adhesive system. Contemp Clin Dent 2013;4:421-6.  Back to cited text no. 8
[PUBMED]  Medknow Journal  
9.
Pavan R, Jain S, Shraddha, Kumar A. Properties and therapeutic application of bromelain: A review. Biotechnol Res Int 2012; 2012:976203.  Back to cited text no. 9
    
10.
Kasraei S, Azarsina M, Khamverdi Z. Effect of Ethylene diamine tetra acetic acid and sodium hypochlorite solution conditioning on microtensile bond strength of one-step self-etch adhesives. J Conserv Dent 2013;16:243-6.  Back to cited text no. 10
[PUBMED]  Medknow Journal  
11.
Inoue G, Nikaido T, Foxton RM, Tagami J. The acid-base resistant zone in three dentin bonding systems. Dent Mater J 2009;28:717-21.  Back to cited text no. 11
    
12.
Jacques P, Hebling J. Effect of dentin conditioners on the microtensile bond strength of a conventional and a self-etching primer adhesive system. Dent Mater 2005;21:103-9.  Back to cited text no. 12
    
13.
Peumans M, Kanumilli P, De Munck J, Van Landuyt K, Lambrechts P, Van Meerbeek B. Clinical effectiveness of contemporary adhesives: A systematic review of current clinical trials. Dent Mater 2005; 21:864-81.  Back to cited text no. 13
    
14.
Braga RR, Meira JB, Boaro LC, Xavier TA. Adhesion to tooth structure: A critical review of "macro" test methods. Dent Mater 2010; 26:e38-49.  Back to cited text no. 14
    
15.
Watanabe I, Nakabayashi N. Measurement methods for adhesion to dentine: The current status in Japan. J Dent 1994;22:67-72.  Back to cited text no. 15
    
16.
Saboia VP, Rodrigues AL, Pimenta LA. Effect of collagen removal on shear bond strength of two single-bottle adhesive systems. Oper Dent 2000;25:395-400.  Back to cited text no. 16
    
17.
Fuentes V, Ceballos L, Osorio R, Toledano M, Carvalho RM, Pashley DH. Tensile strength and microhardness of treated human dentin. Dent Mater 2004;20:522-9.  Back to cited text no. 17
    
18.
Cecchin D, Farina AP, Galafassi D, Barbizam JV, Corona SA, Carlini-Júnior B. Influence of sodium hypochlorite and edta on the microtensile bond strength of a self-etching adhesive system. J Appl Oral Sci 2010;18:385-9.  Back to cited text no. 18
    
19.
Lai SC, Mak YF, Cheung GS, Osorio R, Toledano M, Carvalho RM, et al. Reversal of compromised bonding to oxidized etched dentin. J Dent Res 2001;80:1919-24.  Back to cited text no. 19
    
20.
Barbosa SV, Safavi KE, Spångberg SW. Influence of sodium hypochlorite on the permeability and structure of cervical human dentine. Int Endod J 1994;27:309-12.  Back to cited text no. 20
    
21.
Inaba D, Iijima Y, Takagi O, Ruben J, Arends J. The influence of air-drying on hyper-remineralization of demineralized dentine: A study on bulk as well as on thin wet section of bovine dentine. Caries Res 1995;29:231-6.  Back to cited text no. 21
    
22.
De Castro AK, Hara AT, Pimenta LA. Influence of collagen removal on shear bond strength of one-bottle adhesive systems in dentin. J Adhes Dent 2000;2:271-7.  Back to cited text no. 22
    
23.
Dayem RN. Assessment of the penetration depth of dental adhesives through deproteinized acid-etched dentin using neodymium: Yttrium-aluminum-garnet laser and sodium hypochlorite. Lasers Med Sci 2010;25:17-24.  Back to cited text no. 23
    
24.
Inai N, Kanemura N, Tagami J, Watanabe LG, Marshall SJ, Marshall GW. Adhesion between collagen depleted dentin and dentin adhesives. Am J Dent 1998;11:123-7.  Back to cited text no. 24
    
25.
Ferrari M, Mason PN, Vichi A, Davidson CL. Role of hybridization on marginal leakage and bond strength. Am J Dent 2000;13:329-36.  Back to cited text no. 25
    

Top
Correspondence Address:
Dr. Kirti Chauhan
Department of Conservative Dentistry and Endodontics, College of Dental Sciences, Davangere - 577 004, Karnataka
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-0707.164029

Rights and Permissions



 
 
    Tables

  [Table 1], [Table 2]

This article has been cited by
1 Effect of limonene associated with bioactive glass-ceramic on dentin/adhesive interface
Arthur Henrique Garcia Pinto, Rocio Geng-Vivanco, Fernanda de Carvalho Panzeri Pires-de-Souza
European Journal of Oral Sciences. 2023;
[Pubmed] | [DOI]
2 Comparison of Different Dentin Deproteinizing Agents on Bond Strength and Microleakage of Universal Adhesive to Dentin
Fatih Bedir, Gül Yildiz Telatar
Journal of Advanced Oral Research. 2023; : 2320206823
[Pubmed] | [DOI]
3 Effect of nonthermal atmospheric plasma on bond strength of composite resin using total-etch and self-etch adhesive systems
Nagesh Bolla, AameenaBanu Mayana, PraveenKumar Gali, Sayesh Vemuri, Roopadevi Garlapati, ShaikAfreen Kamal
Journal of Conservative Dentistry. 2023; 26(3): 292
[Pubmed] | [DOI]
4 Effect of nonthermal atmospheric plasma, grape seed extract, and bromelain on immediate bonding of composite to bleached and microabraded surfaces
MayanaAameena Banu, Nagesh Bolla, Sravanthi Tammineedi, Sayesh Vemuri, RamChowdary Basam, AnilKumar Ganapati
Journal of Conservative Dentistry. 2022; 25(1): 42
[Pubmed] | [DOI]
5 Effect of Er:YAG, Co2 lasers, papain, and bromelain enzymes dentin treatment on shear bond strength of composite resin
Farahnaz Sharafeddin, Sara Maroufi
Clinical and Experimental Dental Research. 2022;
[Pubmed] | [DOI]
6 Collagen-depletion strategies in dentin as alternatives to the hybrid layer concept and their effect on bond strength: a systematic review
António H. S. Delgado, Madalena Belmar Da Costa, Mário Cruz Polido, Ana Mano Azul, Salvatore Sauro
Scientific Reports. 2022; 12(1)
[Pubmed] | [DOI]
7 Bromelain: A Potent Phytomedicine
Paridhi Agrawal, Pradnya Nikhade, Aditya Patel, Nikhil Mankar, Shweta Sedani
Cureus. 2022;
[Pubmed] | [DOI]
8 Comparing the Efficacy of Three Minimally Invasive Techniques on Demineralized Dentin in Primary Teeth and Evaluating Its Residual Dentin and Microhardness Levels: An In Vitro Study
Pallavi Urs, S Smitha, R Abinaya, J Janani
International Journal of Clinical Pediatric Dentistry. 2021; 13(6): 585
[Pubmed] | [DOI]
9 Deproteinizing agent, a fore step to better bonding: A literature review
Shubhabrata Pal, Sauvik Galui, Subir Sarkar
International Journal of Pedodontic Rehabilitation. 2021; 0(0): 0
[Pubmed] | [DOI]
10 Antimicrobial Efficacy of a Novel Root Canal Irrigant Made from Coconut and Bromelain Extract against Biofilm Forming Pathogens– An In vitro Evaluation
Jerry Jose, Ajitha Palanivelu
Research Journal of Pharmacy and Technology. 2021; : 5222
[Pubmed] | [DOI]
11 Evaluation of the Effects of Bromelain and Papain Enzymes on Shear Bond Strength of Composite Resin to Enamel
Farahnaz Sharafeddin, Mohammad Hossein Yazdanpanah, Zahra Jowkar, Andrea Scribante
International Journal of Dentistry. 2021; 2021: 1
[Pubmed] | [DOI]
12 Microtensile Bond Strength of Composite Resin Following the Use of Bromelain and Papain as Deproteinizing Agents on Etched Dentin: An In Vitro Study
Roopa R Nadig, Ranjini M Aswathanarayana, Mohd Sibghatullah Khatib, Swapna V Devarasanahalli, Ashwath H Venkateswara
International Journal of Clinical Pediatric Dentistry. 2020; 13(1): 43
[Pubmed] | [DOI]
13 Adeziv sistemlerde güncel yaklasimlar
Nihan CEVLEK, Didem ATABEK
Turkish Journal of Clinics and Laboratory. 2020; 11(3): 193
[Pubmed] | [DOI]
14 Effect of Cavity Disinfectants on Dentin Bond Strength and Clinical Success of Composite Restorations—A Systematic Review of In Vitro, In Situ and Clinical Studies
Ana Coelho, Inês Amaro, Beatriz Rascão, Inês Marcelino, Anabela Paula, José Saraiva, Gianrico Spagnuolo, Manuel Marques Ferreira, Carlos Miguel Marto, Eunice Carrilho
International Journal of Molecular Sciences. 2020; 22(1): 353
[Pubmed] | [DOI]
15 Comparison of Different Dentin Deproteinizing Agents on the Shear Bond Strength of Resin-bonded Dentin
Yogesh Garg, Gyanendra Kumar, Naren Sharma, Kamal Garg, Ramsa Khan, Mohammad Aleemuddin
International Journal of Clinical Pediatric Dentistry. 2020; 13(S1): S69
[Pubmed] | [DOI]
16 Effect of Papain and Bromelain Enzymes on Shear Bond Strength of Composite to Superficial Dentin in Different Adhesive Systems
Farahnaz Sharafeddin, Mina Safari
The Journal of Contemporary Dental Practice. 2019; 20(9): 1077
[Pubmed] | [DOI]



 

Top
 
 
 
  Search
 
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Email Alert *
    Add to My List *
* Registration required (free)  
 


    Abstract
   Introduction
    Materials and Me...
   Results
   Discussion
   Conclusion
    References
    Article Tables

 Article Access Statistics
    Viewed5488    
    Printed124    
    Emailed0    
    PDF Downloaded347    
    Comments [Add]    
    Cited by others 16    

Recommend this journal