Journal of Conservative Dentistry
Home About us Editorial Board Instructions Submission Subscribe Advertise Contact e-Alerts Login 
Users Online: 2302
Print this page  Email this page Bookmark this page Small font sizeDefault font sizeIncrease font size
Year : 2015  |  Volume : 18  |  Issue : 1  |  Page : 44-46

Evaluation of antibacterial and antifungal activity of new calcium-based cement (Biodentine) compared to MTA and glass ionomer cement

1 Department of Conservative Dentistry and Endodontics, Sri Sai College of Dental Surgery, Vikarabad, Andhra Pradesh, India
2 Department of Conservative Dentistry and Endodontics, Saraswati Dhanawantari Dental College, Parbhani, Maharashtra, India
3 Department of Conservative Dentistry and Endodontics, Sri Venkata Sai Institute of Dental Sciences, Mahabubnagar, Telangana, India
4 Department of Prosthodontics, Sri Sai College of Dental Surgery, Vikarabad, Andhra Pradesh, India

Correspondence Address:
Dr. Vankayala Bhavana
Department of Conservative Dentistry and Endodontics, Sri Sai College of dental Surgery, Vikarabad - 501 101, Andhra Pradesh
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0972-0707.148892

Rights and Permissions

Objective: To evaluate the antibacterial and antifungal properties of calcium-based cement, Biodentine (Ca 3 SiO 2 ), compared to commercial glass ionomer cements (GICs) and mineral trioxide aggregate (MTA). Materials and Methods: Pellets of GICs, ProRoot MTA, and Biodentine were prepared to test the influence of these cements on the growth of four oral microbial strains: Streptococcus mutans, Enterococcus faecalis, Escherichia coli, and Candida albicans; using agar diffusion method. Wells were formed by removing the agar and the manipulated materials were immediately placed in the wells. The pellets were lodged in seeded plates and the growth inhibition diameter around the material was measured after 24-72 h incubation at 37°C. The data were analyzed using analysis of variance (ANOVA) test to compare the differences among the three cements at different concentrations. Results: Test indicates that the antimicrobial activity of Biodentine, on all the microorganisms tested, was very strong, showing a mean inhibition zone of 3.2 mm, which extends over time towards all the strains. For Biodentine, GIC, and MTA, the diameters of the inhibition zones for S. mutans were significantly larger than for E. faecalis, Candida, and E. coli (P < 0.05). Conclusion: All materials showed antimicrobial activity against the tested strains except for GIC on Candida. Largest inhibition zone was observed for Streptococcus group. Biodentine created larger inhibition zones than MTA and GIC.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded402    
    Comments [Add]    
    Cited by others 15    

Recommend this journal