A comparative in vitro efficacy of conventional rotatory and chemomechanical caries removal: Influence on cariogenic flora, microhardness, and residual composition
Rene Garcia-Contreras1, Rogelio Jose Scougall-Vilchis2, Rosalia Contreras-Bulnes3, Hiroshi Sakagami4, Raul Alberto Morales-Luckie5, Hiroshi Nakajima6
1 Dental and advanced Studies Research Center "Dr. Keisaburo Miyata"; Deparment of Dental Materials, School of Dentistry, Autonomous University State of Mexico (UAEMex), State of Mexico, Mexico
2 Dental and advanced Studies Research Center "Dr. Keisaburo Miyata"; Deparment of Orthodontics, Autonomous University State of Mexico (UAEMex), State of Mexico, Mexico
3 Dental and advanced Studies Research Center "Dr. Keisaburo Miyata"; Deparment of Pediatric Dentistry, School of Dentistry, Autonomous University State of Mexico (UAEMex), State of Mexico, Mexico
4 Department of Diagnostic and Therapeutic Science, Division of Pharmacology, Meikai University, Saitama, Japan
5 Deparment of Nanomaterials, Center for Research in Sustainable Chemistry (CIQS), School of Chemistry, Autonomous University State of Mexico (UAEMex), State of Mexico, Mexico
6 Biomaterials Science, School of Dentistry, Meikai University, Saitama, Japan
Rogelio Jose Scougall-Vilchis
Department of Orthodontics, Dental and Advanced Studies Research Center, Faculty of Dentistry, Autonomous University State of Mexico, Tollocan-Jesus Carranza, Colonia Universidad, Toluca
Source of Support: None, Conflict of Interest: None
Background: Chemomechanical caries removal system is part of the minimal invasive dentistry; the aim of the study was to compare the amount of bacteria after caries removal with chemomechanical system and conventional rotatory instruments and to test the Vickers microhardness and micro-RAMAN analysis of residual dentin after excavation.
Materials and Methods: Molars were induced for demineralization, confirmed with DIAGNOdent; Streptococcus mutans were inoculated into the cavities and filled. Caries removal was performed with rotatory instruments and chemomechanical system; surviving bacteria were cultured for 24 and 48 hours at 37C. Vickers microhardness and micro-RAMAN analysis were tested after excavation. Data were analyzed with Wilcoxon, continuity correction, odds ratio, ANOVA post hoc Tukey test, and Spearman correlation.
Results: Demineralization was significantly detectable at 240 hours of incubation; conventional rotatory instruments and chemomechanical caries removal were effective in 19.4%-22.6% and 25.8%-32.3%, respectively. Vickers microhardness of chemomechanical system was higher (P < 0.0001) than conventional rotatory instruments and comparable to healthy dentin. Micro-RAMAN analysis showed that healthy dentin is correlated to chemomechanical system (R 2 = 0.683, P < 0.00001) and drilling with burs (R 2 = 0.139, P < 0.00001).
Conclusion: The chemomechanical system is effective for caries elimination, comparable to conventional rotatory instruments; the remaining Vickers microhardness and composition surface tissue are similar to healthy dentin.