Journal of Conservative Dentistry
Home About us Editorial Board Instructions Submission Subscribe Advertise Contact e-Alerts Login 
Users Online: 547
Print this page  Email this page Bookmark this page Small font sizeDefault font sizeIncrease font size
Year : 2013  |  Volume : 16  |  Issue : 5  |  Page : 466-470

Comparative evaluation of the antibacterial effects of four dentine bonding systems: An in vitro study

1 Department of Conservative Dentistry and Endodontics, BBD College of Dental Sciences, Lucknow, India
2 D.A. Pandu Memorial, R.V. Dental College, Bangalore, India

Correspondence Address:
Ruchi Arora
Department of Conservative Dentistry and Endodontics, BBD College of dental sciences, Lucknow
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0972-0707.117503

Rights and Permissions

Aim: The aim of this in vitro study was to evaluate and the compare antibacterial efficacy of four dentin bonding system against Streptococcus mutans, Streptococcus salivarius and Lactobacillus acidophilus over a period of three months using agar disk diffusion test. Methodology: All the three standard bacterial strains were inoculated into BHI broth and incubated at 37°C for 48 hours. A 100 μl of broth suspension containing aliquots of S. mutans, S. salivarius and L. acidophilus were spread onto M-H agar medium using sterile cotton swabs. The experimental groups were as follows: GROUP A: Test conducted for evaluation of antimicrobial efficacy against S. mutans MTCC 497, GROUP B: Test conducted for evaluation of antimicrobial efficacy against S. salivarius MTCC 1938, and GROUP C: Test conducted for evaluation of antimicrobial efficacy against L. acidophilus MTCC 447. For sample preparation, 20 μl bonding agent was dropped with micropipettes on paper disks, and blown dry for 10 seconds. Then it was light-cured at 2 mm for 20 sec using a QTH visible light curing unit. For first reading, the sample disks were placed over the freshly inoculated agar plates and then incubated at 37°C for 48 hours. The rest of the paper disks were stored in dark, submerged in distilled water at 37°C. They were placed on freshly inoculated spread plates after specific time intervals- 1 day, 1 week, 2 weeks, 1 month and 3 months after ageing in PBS. After incubation, the diameters of zones of inhibition around the plates were measured.The experiment was performed twice in triplicate. The data was then statistically analysed using Two way ANOVA test and post hoc. Results: Results showed that Xeno III had the maximum antibacterial efficacy over a period of three months, followed by XP bond. This antibacterial activity was maximum against Streptococcus mutans, followed by Lactobacillus acidophilus and least against Streptococcus salivarius. Adper Easy One and G bond had minimal effect against the test bacteria during the test period. Conclusion: The antibacterial effect decreased over a period of three months for all the dentin bonding systems.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded217    
    Comments [Add]    

Recommend this journal