Journal of Conservative Dentistry
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Year : 2013  |  Volume : 16  |  Issue : 4  |  Page : 331-335

Physical properties and cytotoxicity comparison of experimental gypsum-based biomaterials with two current dental cement materials on L929 fibroblast cells

1 Graduate Dentist, School of Dental Sciences, Universiti Sains Malaysia, Dental Clinic Besar, Kota Bharu, Kelantan, Malaysia
2 Prosthodontics and Biomaterials Unit, School of Dental Sciences, Universiti Sains Malaysia, Kelantan, Malaysia
3 Biomaterials Unit, School of Dental Sciences, Universiti Sains Malaysia, Kelantan, Malaysia

Correspondence Address:
Fazal Reza
School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan
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Source of Support: Short term grant, No. 304/PPSG/61313006 of Universiti Sains Malaysia, Conflict of Interest: None

DOI: 10.4103/0972-0707.114364

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Aim: To evaluate physical properties and cytotoxicity of pure gypsum-based (pure-GYP) and experimental gypsum-based biomaterials mixed with polyacrylic acid (Gyp-PA). The results were compared with calcium hydroxide (CH) and glass ionomer cement (GIC) for application as base/liner materials. Materials and Methods: Vicat's needle was used to measure the setting time and solubility (%) was determined by percentage of weight loss of the materials following immersion in distilled water. For cytotoxicity test, eluates of different concentrations of materials were obtained and pipetted onto L-929 mouse fibroblast cultures and incubated for 3 days. Cellular viability was assessed using Dimethylthiazol diphenyltetrazolium bromide test to determine the cytotoxicity level. Statistical significance was determined by one-way analysis of variance followed by post hoc test ( P < 0.05). Results: Setting time was significantly higher for pure-GYP and Gyp-PA; solubility test showed a similar tendency (pure-Gyp > Gyp-PA > CH = GIC). The pure-Gyp was found as the least cytotoxic materials at different concentrations. At 100 mg/mL dilutions of materials in growth medium highest cytotoxicity was observed with CH group. Conclusion: Cytotoxic effect was not observed with pure-Gyp; application of this novel biomaterial on deeper dentin/an exposed pulp and possibility of gradual replacement of this biodegradable material by dentin like structure would be highly promising.

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