Year : 2017 | Volume
: 20 | Issue : 3 | Page : 147--151
Antimicrobial activity of root canal irrigants against biofilm forming pathogens- An in vitro study
Sheetal Basavraj Ghivari1, Haimanti Bhattacharya2, Kishore G Bhat3, Madhu A Pujar4
1 Department of Conservative Dentistry and Endodontics, KLE VK Institute of Dental Sciences, Belgaum, Karnataka, India
2 Basic Science Research Centre, KLE University, Belgaum, Karnataka, India
3 Department of Microbiology; Department of Molecular Biology and Immunology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belgaum, Karnataka, India
4 Department of Conservative Dentistry and Endodontics, Maratha Mandal Dental College, Belgaum, Karnataka, India
Aims: The aim of the study was to check the antimicrobial activity of the 5% Sodium hypochlorite, 2% Chlorhexidine, 0.10% Octenidine (OCT), and 2% Silver Zeolite (SZ) at different time intervals against a single species biofilm of Enterococcus faecalis, Staphylococcus aureus, and Candida albicans model prepared on a nitrocellulose membrane.
Settings and Design: In vitro nitrocellulose biofilm model was used to check antibacterial efficacy of root canal irrigants.
Materials and Methods: The in vitro nitrocellulose biofilm model was used to check the antibacterial activity of root canal irrigants. Single species biofilms were suspended into 96-well microtiter plate and treated with root canal irrigants for 1, 5, 10, 15, 30, and 60 s, respectively. The remaining microbial load in the form of colony-forming unit/ml after antimicrobial treatment was tabulated and data were statistically analyzed.
Statistical Analysis: SPSS version 17, Kruskal–Wallis ANOVA, Mann–Whitney U-test, and Wilcoxon matched pair test (P < 0.05) were used.
Results: All tested microorganisms were eliminated within 30 s by all the antimicrobial substances tested except normal saline. 2% chlorhexidine and 0.10% OCT were equally effective against C. albicans at 30 s.
Conclusion: The newly tested irrigants have shown considerable antibacterial activity against selected single species biofilm. OCT (0.10%) can be used as an alternative endodontic irrigant.
Sheetal Basavraj Ghivari
4A, Basav Colony, Bauxite Road, Nehru Nagar, Belgaum - 590 010, Karnataka
|How to cite this article:|
Ghivari SB, Bhattacharya H, Bhat KG, Pujar MA. Antimicrobial activity of root canal irrigants against biofilm forming pathogens- An in vitro study.J Conserv Dent 2017;20:147-151
|How to cite this URL:|
Ghivari SB, Bhattacharya H, Bhat KG, Pujar MA. Antimicrobial activity of root canal irrigants against biofilm forming pathogens- An in vitro study. J Conserv Dent [serial online] 2017 [cited 2019 Sep 21 ];20:147-151
Available from: http://www.jcd.org.in/article.asp?issn=0972-0707;year=2017;volume=20;issue=3;spage=147;epage=151;aulast=Ghivari;type=0