Year : 2015 | Volume
: 18 | Issue : 1 | Page : 51--55
Effect of epigallocatechin-3-gallate application for remaining carious dentin disinfection
Jorgiana Silva de Assis, Ramille Araujio Lima, Juliana Paiva Marques Lima, Lidiany Karle Azevedo Rodrigues, Sérgio Lima Santiago
Faculty of Pharmacy, Dentistry and Nursing, Department of Restorative Dentistry, Federal University of Ceará, Fortaleza, Ceará, Brazil
Context: Epigallocatechin-3-gallate (EGCG) is a flavonoid extracted from green tea that demonstrated antimicrobial activity.
Aims: To evaluate the efficacy of EGCG 0.5%, 1%, and 2% concentrations as an antimicrobial solution in dentin caries-like lesions induced in a bacterial-based in vitro model.
Materials and Methods: Twenty-five human dentin specimens were submitted to a microbial-based caries model by immersion in brain heart infusion (BHI) broth inoculated with Streptococcus mutans UA159, for 5 days. After the demineralization period, the specimens were randomly divided into groups: Group I: 0.9% saline solution; Group II: 2% chlorhexidine digluconate; Group III: 0.5% EGCG; Group IV: 1% EGCG; and Group V: 2% EGCG. After the treatments, carious dentin samples were harvested from dentin specimens and analyzed by colony-forming unit (CFU) counts. Data were analyzed by ANOVA and Tukey«SQ»s test.
Results: Log reduction values (SD, CFU.mg -1 ) for Groups I-V were: 5.02 (0.16), 3.96 (0.43), 4.74 (0.26), 4.89 (0.56), and 4.91 (0.40), respectively. There was no statistical difference between the EGCG concentrations and saline solution (P > 0.05). Furthermore, there was no statistical difference between EGCG concentrations (P > 0.05). However, there was a statistically significant difference between the chlorhexidine digluconate group and the other groups (P < 0.05).
Conclusion: EGCG at the studied concentrations were not effective in eliminating S. mutans from dentin caries-like lesions.
Prof. Sérgio Lima Santiago
Rua Monsenhor Furtado, s/n Rodolfo Teófilo, 60.430-355 Fortaleza-CE
|How to cite this article:|
de Assis JS, Lima RA, Marques Lima JP, Azevedo Rodrigues LK, Santiago SL. Effect of epigallocatechin-3-gallate application for remaining carious dentin disinfection.J Conserv Dent 2015;18:51-55
|How to cite this URL:|
de Assis JS, Lima RA, Marques Lima JP, Azevedo Rodrigues LK, Santiago SL. Effect of epigallocatechin-3-gallate application for remaining carious dentin disinfection. J Conserv Dent [serial online] 2015 [cited 2019 Dec 11 ];18:51-55
Available from: http://www.jcd.org.in/article.asp?issn=0972-0707;year=2015;volume=18;issue=1;spage=51;epage=55;aulast=de;type=0