Year : 2014 | Volume
: 17 | Issue : 3 | Page : 271--275
Effect of addition of 2% chlorhexidine or 10% doxycycline on antimicrobial activity of biodentine
Vineeta Nikhil1, Molly Madan2, Charu Agarwal2, Navleen Suri1
1 Department of Conservative Dentistry and Endodontics, Meerut, Uttar Pradesh, India
2 Department of Microbiology Subharti Dental College, Meerut, Uttar Pradesh, India
Aim: The purpose of this in vitro study was to determine whether the addition of 2% chlorhexidine gluconate or 10% doxycycline would enhance the antimicrobial activity of Biodentine against Staphylococcus aureus (ATCC-25923), Enterococcus faecalis (ATCC-29212), Candida albicans (ATCC-90028), and Streptococcus mutans (MTCC-497).
Materials and Methods: Three wells of 4 mm diameter and 4 mm depth on each plate were prepared on the agar medium with standardized suspensions of each microorganism. Biodentine powder mixed with 2% chlorhexidine (0.06 g) or 10% doxycycline (0.30 g) in its liquid or liquid alone was placed to fill each well. Plates were incubated at 37°C as required for microbial growth. A blinded, independent observer measured zones of inhibition. The data were analyzed using independent «DQ»t«DQ» test to compare the differences among the three cement preparations for different micro-organisms.
Results: All Biodentine samples inhibited microbial growth. The highest mean diameters of zone of inhibition for all the micro-organisms were found around Biodentine/chlorhexidine (13.417) followed by Biodentine alone (12.236) and Biodentine/doxycycline (11.25).
Conclusion: In conclusion, adding 2% chlorhexidine gluconate in liquid of Biodentine enhanced the antimicrobial activity of Biodentine against all the tested micro-organisms except Candida albicans, while addition of 10% doxycycline decreased the antimicrobial activity of Biodentine. The differences were significant statistically (P < 0.05).
Department of Conservative Dentistry and Endodontics, Subharti Dental College, NH-58, Delhi - Haridwar Bypass, Meerut - 250 005, Utter Pradesh
|How to cite this article:|
Nikhil V, Madan M, Agarwal C, Suri N. Effect of addition of 2% chlorhexidine or 10% doxycycline on antimicrobial activity of biodentine.J Conserv Dent 2014;17:271-275
|How to cite this URL:|
Nikhil V, Madan M, Agarwal C, Suri N. Effect of addition of 2% chlorhexidine or 10% doxycycline on antimicrobial activity of biodentine. J Conserv Dent [serial online] 2014 [cited 2020 Feb 25 ];17:271-275
Available from: http://www.jcd.org.in/article.asp?issn=0972-0707;year=2014;volume=17;issue=3;spage=271;epage=275;aulast=Nikhil;type=0