Journal of Conservative Dentistry

ORIGINAL ARTICLE
Year
: 2013  |  Volume : 16  |  Issue : 4  |  Page : 331--335

Physical properties and cytotoxicity comparison of experimental gypsum-based biomaterials with two current dental cement materials on L929 fibroblast cells


Nafsiyah Mahshim1, Fazal Reza2, Nor Shamsuria Omar4 
1 Graduate Dentist, School of Dental Sciences, Universiti Sains Malaysia, Dental Clinic Besar, Kota Bharu, Kelantan, Malaysia
2 Prosthodontics and Biomaterials Unit, School of Dental Sciences, Universiti Sains Malaysia, Kelantan, Malaysia

Correspondence Address:
Fazal Reza
School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan
Malaysia

Aim: To evaluate physical properties and cytotoxicity of pure gypsum-based (pure-GYP) and experimental gypsum-based biomaterials mixed with polyacrylic acid (Gyp-PA). The results were compared with calcium hydroxide (CH) and glass ionomer cement (GIC) for application as base/liner materials. Materials and Methods: Vicat«SQ»s needle was used to measure the setting time and solubility (%) was determined by percentage of weight loss of the materials following immersion in distilled water. For cytotoxicity test, eluates of different concentrations of materials were obtained and pipetted onto L-929 mouse fibroblast cultures and incubated for 3 days. Cellular viability was assessed using Dimethylthiazol diphenyltetrazolium bromide test to determine the cytotoxicity level. Statistical significance was determined by one-way analysis of variance followed by post hoc test ( P < 0.05). Results: Setting time was significantly higher for pure-GYP and Gyp-PA; solubility test showed a similar tendency (pure-Gyp > Gyp-PA > CH = GIC). The pure-Gyp was found as the least cytotoxic materials at different concentrations. At 100 mg/mL dilutions of materials in growth medium highest cytotoxicity was observed with CH group. Conclusion: Cytotoxic effect was not observed with pure-Gyp; application of this novel biomaterial on deeper dentin/an exposed pulp and possibility of gradual replacement of this biodegradable material by dentin like structure would be highly promising.


How to cite this article:
Mahshim N, Reza F, Omar NS. Physical properties and cytotoxicity comparison of experimental gypsum-based biomaterials with two current dental cement materials on L929 fibroblast cells.J Conserv Dent 2013;16:331-335


How to cite this URL:
Mahshim N, Reza F, Omar NS. Physical properties and cytotoxicity comparison of experimental gypsum-based biomaterials with two current dental cement materials on L929 fibroblast cells. J Conserv Dent [serial online] 2013 [cited 2019 Sep 21 ];16:331-335
Available from: http://www.jcd.org.in/article.asp?issn=0972-0707;year=2013;volume=16;issue=4;spage=331;epage=335;aulast=Mahshim;type=0