Year : 2012 | Volume
: 15 | Issue : 1 | Page : 22--26
Antimicrobial effect of chlorhexidine digluconate in dentin: In vitro and in situ study
Fátima Maria Cavalcante Borges1, Mary Anne Sampaio de Melo1, Juliana Paiva Marques Lima1, Iriana Carla Junqueira Zanin2, Lidiany Karla Azevedo Rodrigues1
1 Faculty of Pharmacy, Dentistry and Nursing of Federal University of Ceará, Brazil
2 Faculty of Dentistry, Sobral Campus, Federal University of Ceará; Faculty of Dentistry, Federal University of Rio Grande do Sul, Brazil
Aim: The aim of this study was to evaluate a very short-term in vitro and in situ effect of 2% chlorhexidine-digluconate-based (CHX) cavity cleanser on the disinfection of dentin demineralized by cariogenic bacteria.
Materials and Methods: Human dentin slabs were randomly allocated and used in 2 distinct phases, in vitro and in situ, for obtaining demineralized dentin. In vitro, the slabs (n=15) were immersed for 5 days in BHI broth inoculated with Streptococcus mutans CTT 3440. In situ, a double-blind design was conducted in one phase of 14 days, during which 20 volunteers wore palatal devices containing two human dental dentin slabs. On 5 th day in vitro and 14 th day in situ, the slabs were allocated to the two groups: Control group (5 μl of 0.9% NaCl solution) and CHX group (5 μl of 2% chlorhexidine digluconate solution, Cavity Cleanser™ BISCO, Schaumburg, IL, EUA), for 5 minutes. The microbiological analyses were performed immediately before and after the treatments.
Results: The log reductions means found for CHX treatment on tested micro organisms were higher when compared to Control group either in vitro or in situ conditions.
Conclusions: Our results showed that CHX was effective in reducing the cultivable microbiota in contaminated dentin. Furthermore, although the use of chlorhexidine-digluconate-based cavity disinfectant did not completely eliminate the viable microorganisms, it served as a suitable agent to disinfect tooth preparations.
Lidiany Karla Azevedo Rodrigues
Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Department of Operative Dentistry, Cap. Francisco Pedro S/N - Rodolfo Teófilo - Zip Code: 60430-170, Fortaleza-CE
|How to cite this article:|
Borges FC, de Melo MS, Lima JM, Zanin IJ, Rodrigues LA. Antimicrobial effect of chlorhexidine digluconate in dentin: In vitro and in situ study.J Conserv Dent 2012;15:22-26
|How to cite this URL:|
Borges FC, de Melo MS, Lima JM, Zanin IJ, Rodrigues LA. Antimicrobial effect of chlorhexidine digluconate in dentin: In vitro and in situ study. J Conserv Dent [serial online] 2012 [cited 2019 Oct 21 ];15:22-26
Available from: http://www.jcd.org.in/article.asp?issn=0972-0707;year=2012;volume=15;issue=1;spage=22;epage=26;aulast=Borges;type=0