Journal of Conservative Dentistry
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ORIGINAL ARTICLE
Year : 2018  |  Volume : 21  |  Issue : 1  |  Page : 21-25

Cytotoxicity of gypsum-based biomaterial for direct pulp capping using stem cells from human exfoliated deciduous teeth


1 Conservative Dentistry Unit, School of Dental Sciences, Universiti Sains , 16150 Kubang Kerian, Kelantan, Malaysia
2 Biomaterials and Prosthodontics Unit, School of Dental Sciences, Universiti Sains , 16150 Kubang Kerian, Kelantan, Malaysia
3 School of Health Sciences, Universiti Sains , 16150 Kubang Kerian, Kelantan, Malaysia

Correspondence Address:
Dr. Asma Abdullah Nurul
School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan
Malaysia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/JCD.JCD_86_17

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Aim: The aim of this study was to evaluate the cytotoxicity effects of experimental gypsum-based biomaterial prepared with various concentrations of chitosan (Gyp-CHT). Materials and Methods: The study was performed using cell viability assay for mitochondrial dehydrogenase activity in stem cells from human exfoliated deciduous teeth (SHED), after 1, 2, and 3 days of exposure to the biomaterial extracts of varying concentrations. Differences in mean cell viability values were assessed by one-way analysis of variance, followed by Dunnett T3 post hoc test for multiple comparisons (P < 0.05). Results: The cell viability to Gyp-CHT in low extract concentrations was statistically similar to that of the control and different from that of high extract concentrations. Gyp-5% CHT showed the highest percentage of cell viability with 110.92%, 108.56%, and 109.11%. The cell viability showed a tendency toward increment with low extract concentration and no constant effect of CHT on cell viability toward higher or lower. Conclusions: Gyp-CHT biomaterial has no cytotoxic effects on the cultured SHED.


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