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ORIGINAL ARTICLE
Year : 2017  |  Volume : 20  |  Issue : 3  |  Page : 152-156

Cytocompatibility of a self-adhesive gutta-percha root-filling material


1 Department of Cell and Molecular Biology, Fluminense Federal University, Niterói, Brazil
2 Department of Periodontology, Veiga de Almeida University, Rio de Janeiro, Brazil
3 Bioengineering Division, National Institute of Metrology, Quality and Technology, Duque de Caxias, Brazil
4 Department of Endodontics, Grande Rio University, Duque de Caxias, Brazil

Correspondence Address:
Antonio Canabarro
Department of Periodontology, Veiga de Almeida University, Rua Ibituruna 108, Casa 3, Sala 201, CEP 20271-020, Tijuca, Rio de Janeiro, RJ
Brazil
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-0707.218303

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Context: A novel root-filling material based on the incorporation of ultrafine alkaline bioactive glass particles (bioactive gutta-percha, [BGP]) was developed to work without sealer. Aim: In the present study, the objective was to verify the in vitro biological response to this material by assessing its cytocompatibility. Materials and Methods: Prototypes of BGP were compared to conventional gutta-percha (GP), dense polystyrene beads as a negative control and fragments of latex as a positive control. Extracts of each material were prepared according to ISO 10993-5:2009, and human osteoblast-like cells in primary culture were exposed to all extracts for 24 h. Cell viability was assayed sequentially for three different parameters: mitochondrial activity, membrane integrity, and cell density. Statistical Analysis Used: Nonparametric analysis (using Kruskal–Wallis test combined with post hoc Dunn's test) was performed for comparison among groups, with significance established at 5%. Results: BGP reduced mitochondrial activity to 62% of control, but presented no toxicity on membrane integrity and proliferation assays. BGP effect on metabolism was dose-dependent and reduced to acceptable levels with dilution. Conclusion: The novel GP material presented slight dose-dependent effects on cell metabolism but did not affect cell survival.


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