Journal of Conservative Dentistry
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ORIGINAL RESEARCH ARTICLE
Year : 2017  |  Volume : 20  |  Issue : 1  |  Page : 41-45

Additive effect of photoactivated disinfection on the antibacterial activity of QMix2in1 against 6-week Enterococcus faecalis biofilms: An invitro study


1 Department of Conservative Dentistry and Endodontics, K. M. Shah Dental College, Sumandeep Vidyapeeth, Vadodara, Gujarat, India
2 Department of Prosthodontics, Darshan Dental College, Udaipur, Rajasthan, India

Correspondence Address:
Deepa Vaid
Department of Conservative Dentistry and Endodontics, K. M. Shah Dental College, Sumandeep Vidyapeeth, Piparia, Vadodara, Gujarat
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-0707.209072

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Background: Evaluation of the additive effect of photodynamic therapy(PDT) on the antibacterial activity of 2.5% sodium hypochlorite(NaOCl) and QMix against 6-week Enterococcus faecalis biofilms contaminated root canals. Aims: To establish the most suitable irrigant for eradication of 6-week E.faecalis biofilms. Settings and Design: In vitro study. Materials and Methods: A 6-week E.faecalis (ATCC 29212) biofilm was formed in 190 extracted teeth that were subsequently subjected to irrigation protocols as follows. Group A1: normal saline, Group A2: 2.5% NaOCl, Group A3: QMix, Group B1: normal saline and photoactivated disinfection (PAD), Group B2: 2.5% NaOCl and PAD, Group B3: QMix and PAD, Group C: no irrigation. For PAD, irradiation was done three times for 5 s each with 10 s interval on continuous mode with a 980nm diode laser. Samples from the root canals were collected and plated onto brain heart infusion agar plates to determine the colony-forming unit/ml. Statistical Analysis Used: One-way ANOVA, post hoc Tukey's honest significant difference test. Results: Maximum percentage of disinfection(99%) was seen in GroupB2(NaOCl with PDT), which was similar to GroupsA2(97.6%) and B3(98.8%)( P<0.0001). Conclusions: NaOCl with PDT gave maximum disinfection.


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