Journal of Conservative Dentistry
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ORIGINAL RESEARCH ARTICLE
Year : 2016  |  Volume : 19  |  Issue : 5  |  Page : 455-460

Effect of dentin on the antimicrobial efficacy of 3% sodium hypochlorite, 2% chlorhexidine, 17% ethylenediaminetetraacetic acid, and 18% etidronic acid on Candida albicans: An in vitro study


Department of Conservative Dentistry and Endodontics, The Oxford Dental College, Bengaluru, Karnataka, India

Correspondence Address:
Rupali Karale
Department of Conservative Dentistry and Endodontics, The Oxford Dental College, Bengaluru, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-0707.190023

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Aim : The aim of this study was to evaluate the effect of dentin on the antimicrobial efficacy of 3% sodium hypochlorite, 2% chlorhexidine, 17% EDTA and 18% etidronic acid against C.albicans. Methodology: Dentin powder was prepared from mandibular first premolar using electrical grinder and sterilized. 3% NaOCl, 2%CHX, 17% EDTA and 18% etidronic acid were tested against C.albicans in the presence and absence of dentin, in eppendorf tubes. Group 1 (presence of dentin):- 100ul dentin powder + 100ul C.albicans suspension + 100ul irrigating solution. Group 2 (absence of dentin):- 100ul C.albicans suspension+ 100ul irrigating solution. Control group:- 100ul C.albicans suspension.+ 100ul sterile saline Suspension was thoroughly mixed, submitted for serial dilution upto10-5 after 1 min and colony forming units were counted. Results: In group 2 (without dentin powder), 3% NaOCl and 2% CHX showed the lowest bacterial count compared to group 1 (with dentin powder). Dentin had a significant inhibitory effect on 3% NaOCl (P<0.001) and 2% CHX (P<0.001). 17% EDTA showed lowest bacterial count in group 1 (with dentin powder) compared to group 2 (without dentin powder). 18% Etidronic acid showed similar bacterial counts in the both the groups. No reduction was observed in the control group. Conclusion: NaOCl & EDTA showed measurable antimicrobial effect even in the presence of dentin which can be promising in the reduction of C.albicans in root canal therapy.


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