|Year : 2016 | Volume
| Issue : 2 | Page : 166-170
|Shear bond strength evaluation of resin composite bonded to three different liners: TheraCal LC, Biodentine, and resin-modified glass ionomer cement using universal adhesive: An in vitro study
Velagala L Deepa1, Bhargavi Dhamaraju1, Indira Priyadharsini Bollu2, Tandri S Balaji1
1 Department of Conservative Dentistry and Endodontics, Lenora Institute of Dental Sciences, Rajahmundry, Andhra Pradesh, India
2 Department of Conservative Dentistry and Endodontics, St. Joseph Dental College, Andhra Pradesh, India
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|Date of Submission||26-Oct-2015|
|Date of Decision||03-Dec-2015|
|Date of Acceptance||31-Jan-2016|
|Date of Web Publication||14-Mar-2016|
| Abstract|| |
Aims: To compare and evaluate the bonding ability of resin composite (RC) to three different liners: TheraCal LC TM (TLC), a novel resin-modified (RM) calcium silicate cement, Biodentine TM (BD), and resin-modified glass ionomer cement (RMGIC) using an universal silane-containing adhesive and characterizing their failure modes.
Materials and Methods: Thirty extracted intact human molars with occlusal cavity (6-mm diameter and 2-mm height) were mounted in acrylic blocks and divided into three groups of 10 samples each based on the liner used as Group A (TLC), Group B (BD), and Group C (RMGIC). Composite post of 3 mm diameter and 3 mm height was then bonded to each sample using universal adhesive. Shear bond strength (SBS) analysis was performed at a cross-head speed of 1 mm/min.
Statistical Analysis Used: Statistical analysis was performed with one-way analysis of variance (ANOVA) and post hoc test using Statistical Package for the Social Sciences (SPSS) version 20.
Results: No significant difference was observed between group A and group C (P = 0.573) while group B showed the least bond strength values with a highly significant difference (P = 0.000). The modes of failure were predominantly cohesive in Groups A and B (TLC and BD) while RMGIC showed mixed and adhesive failures.
Conclusions: Hence, this present study concludes that the bond strength of composite resin to TLC and RMGIC was similar and significantly higher than that of BD following application of universal adhesive.
Keywords: Biodentine TM (BD); failure modes; resin-modified glass ionomer; stereomicroscope; TheraCal LC TM (TLC); universal adhesive
|How to cite this article:|
Deepa VL, Dhamaraju B, Bollu IP, Balaji TS. Shear bond strength evaluation of resin composite bonded to three different liners: TheraCal LC, Biodentine, and resin-modified glass ionomer cement using universal adhesive: An in vitro study. J Conserv Dent 2016;19:166-70
|How to cite this URL:|
Deepa VL, Dhamaraju B, Bollu IP, Balaji TS. Shear bond strength evaluation of resin composite bonded to three different liners: TheraCal LC, Biodentine, and resin-modified glass ionomer cement using universal adhesive: An in vitro study. J Conserv Dent [serial online] 2016 [cited 2019 Aug 25];19:166-70. Available from: http://www.jcd.org.in/text.asp?2016/19/2/166/178696
| Introduction|| |
Extensive research has been taking place in generating bioactive restorative materials with a potential for remineralization.  Bioactivity refers to apatite-forming ability  while biomineralization is the ability to get anchored to the underlying dentin by the formation of a mineral-rich interfacial layer and a tag-like structure extending from the interfacial layer to the dentinal tubules.  Biodentine TM (BD) (Septodont, Saint-Maur-des-Fossιs, Creteil, France) and TheraCal LC TM (Bisco Inc, Schamburg, IL, USA) are calcium silicate-based bioactive liners that are proposed as alternatives to glass ionomers (GIs).
The use of bioactive liners beneath resin composite (RC) would clinically be more advantageous than using GI liners as they are biologically well-tolerated by the pulp tissue  and have comparatively higher remineralizing ability.  The success of these laminate restorations depend not only on the bond strength of the liner to the dentin but also on the quality of bond between liner and overlying RC. Various studies suggest the application of resin-modified glass ionomer cement (RMGIC) instead of GI in the sandwich technique because of improved bond strength to RC due to its chemical bonding. , The bond strength of RMGIC to RC varies depending on the type of adhesive used and it has been proved that self-etch is better than total etch. 
Biodentine TM (BD) is recently being used as a dentin replacement material under composite restorations.  A study by Danya F. Hashem et al.  showed no significant difference in bond strength between BD and RC in either self-etch or total etch mode. They also suggested BD composite restoration is a two-stage clinical procedure that requires a minimum waiting period of 2 weeks for adequate maturation of the BD to attain the physicomechanical properties sufficient enough to withstand the contraction forces of RC.
TheraCal LC TM (TLC) is a novel light-cured mineral trioxide aggregate (MTA)-filled, resin-modified (RM) calcium silicate cement and was given approval as a liner under composite restorations aiming to achieve a bond between the different layers of materials and as a pulp protectant. Gandolfi et al.  studied the chemical and physical properties of TLC and reported more calcium release than ProRoot MTA and Dycal. It was reported that calcium silicate-based materials showed apatite formation at a faster rate than calcium hydroxide-based materials.  However, there are contradictory findings reported in the literature about the hydration characteristics of TLC.  The role of moisture drawn in from the pulp and dentin is also unclear. TLC shows physiochemical bonding to the dentin and is well-tolerated by immortalized odontoblast cells.  Recently, Cantekin  proved that the bond strength of TheraCal methacrylate-based composite was significantly higher than that with silorane-based composites and GI cement. Currently, there is limited information in the literature on the bonding ability of TLC to RC in comparison to other liners.
Recently, a new single bottle universal or multimode adhesive with silanes (Single Bond Universal TM , 3M ESPE, St. Paul, MN, USA) was introduced that simplifies the bonding procedure as single adhesive and can be used in self-etch or total etch or selective etch mode and on any surfaces (enamel, dentin, any direct, or indirect restorative materials) without additional primer.
No study till date has compared the bonding ability between TLC, BD, and RMGIC to RC using universal adhesive. Hence, in the present study the shear bond strength (SBS) of BD/TLC/RMGIC to composite using universal adhesive was compared and the null hypothesis was that there is no difference in the SBS within each substrate (TLC/BD/RM-GIC). The study also aimed to identify the specific modes of failure.
| Materials and methods|| |
The materials used are shown in [Table 1]. Thirty human intact molars extracted for periodontal reasons were collected for the study and the teeth were cleaned with ultrasonic scalers and stored in saline. The occlusal surfaces were grinded perpendicular to the long axis of the tooth with a high-speed diamond disc to obtain a flat surface. Then a cavity of 6 mm width and 2 mm depth was prepared to retain the liner. These teeth were mounted in acrylic resin blocks using a rectangular aluminium mould that was 15 mm/25 mm in dimension such that the occlusal surfaces were flush with the resin surface. These 30 samples were randomly divided into three groups: Group A - TLC; (TheracalLC TM , Bisco Inc, Schamburg, IL, USA), Group B - BD; (Biodentine TM , Septodont, Saint-Maur-des-Fossιs, Creteil, France) and Group C- RMGIC; (Fuji II LC TM ,GC Corporation, Tokyo, Japan) and the cavities were filled as per manufacturer's instructions [Table 1] and their surfaces were not finished to mimic the clinical scenario.
Universal adhesive, (Single Bond Universal TM , 3M ESPE, St. Paul, MN, USA) was applied on TLC/BD/RM-GIC surface with a bristle brush, rubbed for 20 s followed by gentle air drying with oil-free compressed air for approximately 5 s to evaporate the solvent and was light cured for 10 s after placing the polyethylene tube (2-mm diameter, 2-mm height) as per the manufacturer's instructions.
RC (Filtek TM Z-350 XT, 3M ESPE, St. Paul, MN, USA) was placed in the tube and light-cured with a light-emitting diode light-curing unit (Bluephase, Ivoclar Vivadent, Schaan, Lichtenstein) with an intensity of 1,200 mV/cm 2 for 20 s. After the completion of RC build-up, the polyethylene tubes were removed with a sharp knife. All specimens were stored at 37C in water for 24 h.
Measurement of shear bond strength
The specimens were attached to the universal testing machine (Instron 8500, Instron Corporation, Canton, OH, USA). A chisel with knife edge was gently held flush against the RC - TLC/BD/RM-GIC interface and loaded at a cross-head speed of 1.0 mm/min until bond failure occurred. The load at failure was recorded in N/mm square and then converted to MPa.
The fractured test specimens were examined under a stereomicroscope (Swift Stereo SM80, Tokyo, Japan) at a magnification of × 25 and fractures were classified as follows: Cohesive failure - Failure with in TLC/BD/RMGIC or RC, adhesive failure - Failure at RC- TLC/BD/RM-GIC interface, and mixed failure - When two modes of failure occur simultaneously. Fracture analysis was performed by a single observer who was completely uninformed about the experimental groups.
Statistical analysis was performed by using MS Excel 2007 and Statistical Package for the Social Sciences (SPSS) version 20.0 (SPSS Inc, Chicago, USA). Descriptive statistical data was presented in the form of mean and standard deviation. One-way analysis of variance (ANOVA) was performed to assess the mean significant difference between the different groups and LSD post hoc tests were used for multiple group comparisons. P value less than 0.05 was considered to be statistically significant.
| Results|| |
The mean SBS values and standard deviations (SDs) are shown in [Table 2] and were analyzed using ANOVA test; the post hoc test was used for intergroup comparison. Groups A and C (TLC, RMGIC) in comparison to Group B (BD) showed a very high significant difference (P = 0.000). Group A (TLC) showed no significant difference in bond strength compared to Group C (RMGIC) (P = 0.573). Group B (BD) showed the least bond strength values. The observed modes of failure were predominantly cohesive in Groups A and B (TLC and BD) while RMGIC showed mixed and adhesive failures [Figure A [Additional file 1]].
| Discussion|| |
TLC/BD releases calcium and silicon ions into the underlying dentin. , According to Saito et al.  silica is a stronger inducer for dentin matrix remineralization than fluoride ions of RMGIC. Cytotoxicity studies showed that TLC/BD is well-tolerated by immortalized odontoblast cells. These are the cells that retained their ability to divide with stable phenotypic protein expression profiles and ability to produce mineralized dentin extracellular matrix under in vitro conditions. , The conclusions from these studies bear relevance to the use of TLC/BD as a liner and an alternative to RMGIC in laminate restorations, provided the bond to composite is adequate to withstand polymerization stresses (at least 17 MPa).  Bond strength between TLC/BD/RMGIC liners and composite depends on their physicochemical properties, nature of the bond between liner and RC, and the types of adhesive used.
In the present study, methacryloyloxydecyl dihydrogen phosphate (MDP)-based, universal adhesive with silanes was selected. This self-etch 10-MDP-based adhesive shows chemical bonding to Ca ions, and Al and zirconium oxides. , The bifunctional silane molecule bonds chemically to silica-containing materials and has methacrylate functionality that allows chemical union with resinous substrate. Silanes also act as adhesion promoters by enhancing the wetting ability of th eadhesive system.  This adhesive was selected in our study, aiming for additional chemical bonding with Ca releasing bio active liners.
In this study, Groups A and C (TLC and RM-GIC) showed significantly higher bond strengths than Group B (BD) as TLC and RMGIC are resin-based light cure cements that attain early cohesive strength on photo activation. Group B (BD) showed the least SBS means (5.666 MPa), which may have been due to low early strength of the material per se and this was in agreement with previous studies.  BD is a porous material that needs at least 2 weeks time for crystallization of hydrated calcium silicate gel to attain bulk strength adequate to withstand the polymerization stresses.  In the present study, bonding was performed to BD immediately after 12 min to depict a single appointment clinical procedure. This could be the reason for low bond strength and cohesive failures in BD.
Groups A and C (TLC and RM-GIC) showed statistically similar SBS means (18.249 and 18.656 MPa, respectively) and were adequate to withstand contraction stresses of RC. This could be due to similar resin chemistry promoting chemical adhesion with RC as proposed for RMGIC. Hydroxyethyl methacrylate (HEMA) incorporated into the TLC and RM-GIC forms a chemical bond with the resin of the composite. Additional chemical union is due to copolymerization of unreacted methacrylate groups present in the oxygen-inhibited layer of TLC/RMGIC with those of composite resin. , The resin bonding agent intermixes with both composite and TLC/RMGIC by true chemical bonding to create a strong interface.
Though the SBS of Groups A and C (TLC and RMGIC) were similar, the failure modes were predominantly cohesive in Group A (TLC) while Group C (RMGIC) showed 60% mixed failures and 40% adhesive failures. Cohesive failure in TLC could have been due to its low bulk strength. TLC, a resin-modified (RM) calcium silicate cement is a combination of a HEMA/TEGDMA-based resin and calcium-silicate powder. On light activation, HEMA and TEGDMA monomers create a polymeric network that is able to stabilize the outer surface of the cement. Thus formed poly-HEMA is hydrophilic and favors the absorption of moisture and triggers a second setting reaction that is hydration of calcium silicate particles with liberation of calcium ions.  TLC releases more Ca ions than RMGIC. Hence, a strong chemical bonding among adhesive and Ca, Al, Zr, and silicon ions of TLC could have resulted in similar bond strength values as RMGIC group in spite of its low bulk strength.
| Conclusion|| |
Within the limitations of this in vitro study, the following conclusions are made:
- RMGIC and TLC achieved adequate bond strength to withstand contraction forces from overlying composite resin due to the presence of a resin matrix.
- Composite restoration can be placed immediately over TLC and RMGIC, completing the procedure in single appointment.
- BD showed significantly lower bond strength values when immediately bonded to RC. The mode of failure was cohesive within BD, indicating it as a weak material in its early setting phase.
- This highlights the importance of leaving BD to mature for longer time period before the application of overlying composite restoration.
We would like to thank Mr. Govinda Raju, In-Charge, MSME Testing Station, Hyderabad, India for letting out the institute's facilities for bond strength testing, and Mr. Ganapati, Assistant Professor of Community Medicine, GSL Medical College, Rajahmundry, Andhra Pradesh, India for helping in statistical analysis.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Watson TF, Atmeh AR, Sajini S, Cook RJ, Festy F. Present and future of glass-ionomers and calcium-silicate cements as bioactive materials in dentistry: Biophotonics-based interfacial analyses in health and disease. Dent Mater 2014;30:50-61.
Gandolfi MG, Taddei P, Siboni F, Modena E, Ciapetti G, Prati C. Development of the foremost light-curable calcium-silicate MTA cement as root-end in oral surgery. Chemical-physical properties, bioactivity and biological behaviour. Dent Mater 2011;27:e134-57.
Reyes-Carmona JF, Felippe MS, Felippe WT. Biomineralization ability and interaction of mineral trioxide aggregate and white Portland cement with dentin in a phosphate-containing fluid. J Endod 2009;35:731-6.
Cannon M, Gerodias N, Viera A, Percinoto C, Jurado R. Primate pulpal healing after exposure and TheraCal application. J Clin Pediatr Dent 2014;38:333-7.
Saito T, Toyooka H, Ito S, Crenshaw MA. In vitro
study of remineralization of dentin: Effects of ions on mineral induction by decalcified dentin matrix. Caries Res 2003;37:445-9.
Li J, Liu Y, Liu Y, Söremark R, Sundström F. Flexure strength of resin-modified glass ionomer cements and their bond strength to dental composites. Acta Odontol Scand 1996;54:55-8.
Farah CS, Orton VG, Collard SM. Shear bond strength of chemical and light-cured glass ionomer cements bonded to resin composites. Aust Dent J 1998;43:81-6.
Arora V, Kundabala M, Parolia A, Thomas MS, Pai V. Comparison of the shear bond strength of RMGIC to a resin composite using different adhesive systems: An in vitro
study. J Conserv Dent 2010;13:80-3.
Camilleri J. Investigation of biodentine as dentine replacement material. J Dent 2013;41:600-10.
Hashem DF, Foxton R, Manoharan A, Watson TF, Banerjee A. The physical characteristics of resin composite-calcium silicate interface as part of a layered/laminate adhesive restoration. Dent Mater 2014;30: 343-9.
Gandolfi MG, Siboni F, Prati C. Chemical-physical properties of TheraCal, a novel light-curable MTA-like material for pulp capping. Int Endod J 2012;45:571-9.
Gandolfi MG, Siboni F, Botero T, Bossù M, Riccitiello F, Prati C. Calcium silicate and calcium hydroxide materials for pulp capping: Biointeractivity, porosity, solubility and bioactivity of current formulations. J Appl Biomater Funct Mater 2015;13:43-60.
Camilleri J. Hydration characteristics of biodentine and theracal used as pulp capping materials. Dent Mater 2014;30:709-15.
Hebling J, Lessa FC, Nogueira I, Carvalho RM, Costa CA. Cytotoxicity of resin-based light-cured liners. Am J Dent 2009;2:137-42.
Cantekin K. Bond strength of different restorative materials to light-curable mineral trioxide aggregate. J Clin Pediatr Dent 2015;39:143-8.
Han L, Okiji T. Uptake of calcium and silicon released from calcium silicate-based endodontic materials into root canal dentine. Int Endod J 2011;44:1081-7.
Davidson CL, de Gee AJ, Feilzer A. The competition between the composite-dentin bond strength and the polymerization contraction stress. J Dent Res 1984;63:1396-9.
Yoshida Y, Nagakane K, Fukuda R, Nakayama Y, Okazaki M, Shintani H, et al
. Comparative study on adhesive performance of functional monomers. J Dent Res 2004;83:454-8.
Blatz MB, Sadan A, Kern M. Resin - ceramic bonding: A review of the literature. J Prosthet Dent 2003;89:268-74.
Söderholm KJ, Shang SW. Molecular orientation of silane at the surface of colloidal silica. J Dent Res 1993;72:1050-4.
Bachoo IK, Seymour D, Brunton P. A biocompatible and bioactive replacement for dentine: Is this a reality? The properties and uses of a novel calcium-based cement. Br Dent J 2013;214:E5.
Kerby RE, Knobloch L. The relative shear bond strength of visible light-curing and chemically curing glass-ionomer cement to composite resin. Quintessence Int 1992;23:641-4.
Dr. Velagala L Deepa
Department of Conservative Dentistry and Endodontics, Lenora Institute of Dental Sciences, Rajanagaram, Rajahmundry, Andhra Pradesh
Source of Support: None, Conflict of Interest: None
[Table 1], [Table 2]
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