Journal of Conservative Dentistry
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Year : 2014  |  Volume : 17  |  Issue : 3  |  Page : 271-275

Effect of addition of 2% chlorhexidine or 10% doxycycline on antimicrobial activity of biodentine

1 Department of Conservative Dentistry and Endodontics, Meerut, Uttar Pradesh, India
2 Department of Microbiology Subharti Dental College, Meerut, Uttar Pradesh, India

Correspondence Address:
Vineeta Nikhil
Department of Conservative Dentistry and Endodontics, Subharti Dental College, NH-58, Delhi - Haridwar Bypass, Meerut - 250 005, Utter Pradesh
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0972-0707.131795

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Aim: The purpose of this in vitro study was to determine whether the addition of 2% chlorhexidine gluconate or 10% doxycycline would enhance the antimicrobial activity of Biodentine against Staphylococcus aureus (ATCC-25923), Enterococcus faecalis (ATCC-29212), Candida albicans (ATCC-90028), and Streptococcus mutans (MTCC-497). Materials and Methods: Three wells of 4 mm diameter and 4 mm depth on each plate were prepared on the agar medium with standardized suspensions of each microorganism. Biodentine powder mixed with 2% chlorhexidine (0.06 g) or 10% doxycycline (0.30 g) in its liquid or liquid alone was placed to fill each well. Plates were incubated at 37°C as required for microbial growth. A blinded, independent observer measured zones of inhibition. The data were analyzed using independent "t" test to compare the differences among the three cement preparations for different micro-organisms. Results: All Biodentine samples inhibited microbial growth. The highest mean diameters of zone of inhibition for all the micro-organisms were found around Biodentine/chlorhexidine (13.417) followed by Biodentine alone (12.236) and Biodentine/doxycycline (11.25). Conclusion: In conclusion, adding 2% chlorhexidine gluconate in liquid of Biodentine enhanced the antimicrobial activity of Biodentine against all the tested micro-organisms except Candida albicans, while addition of 10% doxycycline decreased the antimicrobial activity of Biodentine. The differences were significant statistically (P < 0.05).

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