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ORIGINAL ARTICLE
Year : 2014  |  Volume : 17  |  Issue : 2  |  Page : 124-128

Cut off values of laser fluorescence for different storage methods at different time intervals in comparison to frozen condition: A 1 year in vitro study


1 Departments of Conservative & Endodontics, Institute of Dental Sciences, Jammu and Kashmir, India
2 Departments of Oral Medicine and Radiology, Government Dental College, Srinagar, Jammu and Kashmir, India
3 Departments of Conservative Dentistry & Endodontics, Government Dental College, Srinagar, Jammu and Kashmir, India
4 Departments of Oral and Maxillofacial Surgery, Government Dental College, Srinagar, Jammu and Kashmir, India
5 Departments of Conservative & Endodontics, Government Dental College, Jammu and Kashmir, India

Correspondence Address:
Rudra Kaul
Department of Conservative and Endodontics, Institute of Dental Sciences, Jammu and Kashmir
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-0707.128043

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Aims: The aim of the following study is to evaluate the change in laser fluorescence (LF) values for extracted teeth stored in different solutions over 1 year period, to give cut-off values for different storage media at different time intervals to get them at par with the in vivo conditions and to see which medium gives best results with the least change in LF values and while enhancing the validity of DIAGNOdent in research. Materials and Methods: Ninety extracted teeth selected, from a pool of frozen teeth, were divided into nine groups of 10 each. Specimens in Groups 1-8 were stored in 1% chloramine, 10% formalin, 10% buffered formalin, 0.02% thymol, 0.12% chlorhexidine, 3% sodium hypochlorite, a commercially available saliva substitute-Wet Mouth (ICPA Pharmaceuticals) and normal saline respectively at 4°C. The last group was stored under frozen condition at −20°C without contact with any storage solution. DIAGNOdent was used to measure the change the LF values at day 30, 45, 60, 160 and 365. Statistical Analysis Used: The mean change in LF values in different storage mediums at different time intervals were compared using two-way ANOVA. Results: At the end of 1 year, significant decrease in fluorescence (P < 0.05) was observed in Groups 1-8. Maximum drop in LF values occurred between day 1 and 30. Group 9 (frozen specimens) did not significantly change their fluorescence response. Conclusions: An inevitable change in LF takes place due to various storage media commonly used in dental research at different time intervals. The values obtained from our study can remove the bias caused by the storage media and the values of LF thus obtained can hence be conveniently extrapolated to the in vivo condition.


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