Journal of Conservative Dentistry
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Year : 2012  |  Volume : 15  |  Issue : 1  |  Page : 32-35

Comparative evaluation of apical extrusion of bacteria using hand and rotary systems : An in vitro study

Department of Conservative Dentistry and Endodontics, Sharad Pawar Dental College, Sawangi (Meghe), Wardha, Maharashtra, India

Correspondence Address:
Sheetal B Ghivari
Department of Conservative Dentistry and Endodontics, Sharad Pawar Dental College, Sawangi (M), Wardha, Maharashtra - 442 004
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0972-0707.92603

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Aim: To evaluate the bacteria extruded apically during root canal preparation using two hand and rotary instrumentation techniques. Materials and Methods: Eighty freshly extracted mandibular premolars were mounted in bacteria collection apparatus. Root canals were contaminated with the pure culture of Enterococcus fecalis (ATCC 29212) and dried at 37°C for 24 h. Bacteria extruded were collected, incubated in brain heart infusion agar for 24 h at 36°C and the colony forming units (CFU) were counted. Statistical Analysis: The mean number of colony forming units were calculated by One-way ANOVA and comparison between the groups made by multiple comparison (Dunnet D) test. Results: The step-back technique extruded highest number of bacteria in comparison to other hand and rotary Ni-Ti systems. Conclusion: Under the limitation of this study all hand and rotary instrumentation techniques extruded bacteria. Among all the instrumentation techniques step-back technique extruded more number of bacteria and K-3 system the least. Further in vivo research in this direction could provide more insight into the biologic factors associated and focus on bacterial species that essentially play a major role in post instrumentation flare-ups.

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