Journal of Conservative Dentistry
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Year : 2012  |  Volume : 15  |  Issue : 1  |  Page : 22-26

Antimicrobial effect of chlorhexidine digluconate in dentin: In vitro and in situ study

1 Faculty of Pharmacy, Dentistry and Nursing of Federal University of Ceará, Brazil
2 Faculty of Dentistry, Sobral Campus, Federal University of Ceará; Faculty of Dentistry, Federal University of Rio Grande do Sul, Brazil

Correspondence Address:
Lidiany Karla Azevedo Rodrigues
Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Department of Operative Dentistry, Cap. Francisco Pedro S/N - Rodolfo Teófilo - Zip Code: 60430-170, Fortaleza-CE
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Source of Support: This research received the financial support of Grant # 477070/2008-6 from National Council for Scientific and Technological Development (CNPq). This paper was based on a thesis submitted by the first author to the Faculty of Pharmacy, Dentistry and Nursing of Federal University of Ceará, in partial fulfillment of the requirements for a MS degree in Dentistry., Conflict of Interest: None

DOI: 10.4103/0972-0707.92601

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Aim: The aim of this study was to evaluate a very short-term in vitro and in situ effect of 2% chlorhexidine-digluconate-based (CHX) cavity cleanser on the disinfection of dentin demineralized by cariogenic bacteria. Materials and Methods: Human dentin slabs were randomly allocated and used in 2 distinct phases, in vitro and in situ, for obtaining demineralized dentin. In vitro, the slabs (n=15) were immersed for 5 days in BHI broth inoculated with Streptococcus mutans CTT 3440. In situ, a double-blind design was conducted in one phase of 14 days, during which 20 volunteers wore palatal devices containing two human dental dentin slabs. On 5 th day in vitro and 14 th day in situ, the slabs were allocated to the two groups: Control group (5 μl of 0.9% NaCl solution) and CHX group (5 μl of 2% chlorhexidine digluconate solution, Cavity Cleanser™ BISCO, Schaumburg, IL, EUA), for 5 minutes. The microbiological analyses were performed immediately before and after the treatments. Results: The log reductions means found for CHX treatment on tested micro organisms were higher when compared to Control group either in vitro or in situ conditions. Conclusions: Our results showed that CHX was effective in reducing the cultivable microbiota in contaminated dentin. Furthermore, although the use of chlorhexidine-digluconate-based cavity disinfectant did not completely eliminate the viable microorganisms, it served as a suitable agent to disinfect tooth preparations.

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