Biocompatibility assessment of pastes containing Copaiba oilresin, propolis, and calcium hydroxide in the subcutaneous tissue of rats
Lucas Garcia1, Santos Cristiane2, Mestriner Jr. Wilson2, Mestriner Jr. Soraya2, Lopes A Ruberval3, Ramos Mônica4, de Freitas Osvaldo5
1 Department of Dental Materials and Prosthodontics, Dental School of Ribeirão Preto, University of São Paulo, Brazil
2 Department of Clinical Child, Social and Preventive Dentistry, Ribeirao Preto School of Dentistry, University of Sao Paulo, Brazil
3 Department of Morphology, Stomatology, and Physiology, Ribeirao Preto School of Dentistry, University of Sao Paulo, Brazil
4 School of Pharmaceutical Sciences, Federal University of Rio de Janeiro, Osvaldo Cruz Foundation, SP, Brazil
5 Department of Pharmaceutical Sciences, Ribeirao Preto School of Pharmaceutical Sciences, University of Sao Paulo, Ribeirao Preto, SP, Brazil
Rua Bernardino de Campos, 1002, apto. 30 - CEP 14015-130, Ribeirao Preto, SP
Source of Support: None, Conflict of Interest: None
Aim : To assess the biocompatibility of two endodontic pastes based on calcium hydroxide and propolis, using two vehicles - non-fractionated Copaiba-oilresin (A) and volatile fraction of Copaiba-oilresin (B), in the connective tissue of rats.
Materials and Methods : Fifteen rats had four polyethylene tubes implanted in their backs; each pair of tubes contained one of the pastes. The tube side was considered the control. After 7, 21, and 42 days, the animals were euthanized.
Results : The inflammatory reaction was moderate at seven days for A and severe for B. At 21 days, it was slight for A and moderate for B; and at 42 days, it was slight for A and B. Tissue reaction ranged from slight (7 / 21 days) to no inflammation (42 days) for the control group. Statistical analysis (Kruskal-Wallis test, P < 0.01) demonstrated no significant difference between the pastes and control group (P > 0.01).
Conclusion : Both pastes presented satisfactory tissue reaction in the connective tissue of rats.